Facile hydrothermal synthesis of branched polyethylenimine grafted electrospun polyacrylonitrile fiber membrane as a highly efficient and reusable bilirubin adsorbent in hemoperfusion

Rui Zhao; Yumei Li; Xiang Li; Yanzi Li; Bolun Sun; Shen Chao; Ce Wang

doi:10.1016/j.jcis.2017.12.059

Facile hydrothermal synthesis of branched polyethylenimine grafted electrospun polyacrylonitrile fiber membrane as a highly efficient and reusable bilirubin adsorbent in hemoperfusion

J Colloid Interface Sci. 2018 Mar 15:514:675-685. doi: 10.1016/j.jcis.2017.12.059. Epub 2017 Dec 22.

Authors

Rui Zhao¹, Yumei Li², Xiang Li³, Yanzi Li¹, Bolun Sun¹, Shen Chao¹, Ce Wang⁴

Affiliations

¹ Alan G. MacDiarmid Institute, College of Chemistry, Jilin University, Changchun 130012, PR China.
² Alan G. MacDiarmid Institute, College of Chemistry, Jilin University, Changchun 130012, PR China; Department of Clinical Pharmacy and Traditional Chinese Medicine Pharmacology, School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, Changchun 130117, PR China.
³ Alan G. MacDiarmid Institute, College of Chemistry, Jilin University, Changchun 130012, PR China. Electronic address: xiangli@jlu.edu.cn.
⁴ Alan G. MacDiarmid Institute, College of Chemistry, Jilin University, Changchun 130012, PR China. Electronic address: cwang@jlu.edu.cn.

PMID: 29310097
DOI: 10.1016/j.jcis.2017.12.059

Abstract

Bilirubin is a pathogenic toxin in the blood of patients with liver failure, and bilirubin adsorbents have been applied to remove the extra bilirubin in hemoperfusion. However, the developing of high-performance adsorbents with satisfying removal ability, physical/chemical stability, biocompatibility and hemocompatibility still needs to be explored. In this study, branched polyethylenimine (bPEI) grafted electrospun polyacrylonitrile (PAN) fiber membrane (bPEIPANFM) was fabricated via a green hydrothermal process. The hydrothermal temperature, hydrothermal time and the weight ratio of bPEI to PAN were investigated to seek the optimal bilirubin adsorption capacity. The results revealed that bPEIPANFM (hydrothermal temperature at 160 °C, hydrothermal time at 10 h and the weight ration of bPEI to PAN at 10:1) showed the best removal ability toward bilirubin. Batch adsorption experiments for both free bilirubin solution and bovine serum albumin (BSA)-bonded bilirubin solution were studied. The maximum adsorption capacity of bPEIPANFM toward BSA-bonded bilirubin was 112.87 mg/g, which is higher than those of many reported adsorbents. After ten regeneration cycles, the removal efficiency could still maintain 95%. Moreover, the bPEIPANFM also exhibited excellent biocompatibility and hemocompatibility. These obtained results demonstrate that this study may provide a new adsorbent for removing bilirubin in hemoperfusion applications.

Keywords: Adsorption; Bilirubin; Electrospun fiber membrane; Hemocompatibility; Hydrothermal grafting.