Liposome-Assisted Metabolic Glycan Labeling With Cell and Tissue Selectivity

Abstract

Metabolic labeling of glycans with sugar chemical reporters (i.e., unnatural sugars bearing a bioorthogonal group), followed by bioorthogonal reaction with imaging probes or affinity tags, has enabled visualization and proteomic analysis of glycosylation in live cells and in living animals. This two-step metabolic glycan labeling strategy has emerged as a powerful tool for probing glycosylation, but suffers from a lack of cell-type selectivity. Here we describe liposome-assisted bioorthogonal reporter (LABOR), a liposome-assisted format of metabolic glycan labeling that allows for cell-selective and tissue-specific glycan imaging and glycoproteomic profiling. After a brief introduction of the principles and applications of LABOR, we provide detailed protocols for performing LABOR in cell culture and in living mice.

Keywords: 9-Azido sialic acid; Brain targeting; Cell selectivity; Fluorescence imaging; Glycoproteomics; LABOR; Liposome-assisted labeling; Metabolic glycan labeling; Tissue specificity.