Abstract
We proposed a terminal deoxynucleotidyl transferase (TdT)-assisted rolling circle amplification (RCA) strategy for the amplified detection of genome-containing biological targets. The synergetic signal amplification of DNase I-mediated genomic DNA fragmentation and subsequent RCA allow the sensing platform to have extraordinarily high sensitivity.
MeSH terms
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Benzothiazoles
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Biosensing Techniques
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DNA / chemistry*
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DNA / genetics
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DNA Nucleotidylexotransferase / chemistry
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DNA Nucleotidylexotransferase / metabolism*
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DNA-Directed DNA Polymerase
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Fluorescent Dyes
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Genome
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Nucleic Acid Amplification Techniques / methods*
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Thiazoles / chemistry
Substances
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Benzothiazoles
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Fluorescent Dyes
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Thiazoles
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thioflavin T
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DNA
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DNA Nucleotidylexotransferase
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DNA-Directed DNA Polymerase