Amplified detection of genome-containing biological targets using terminal deoxynucleotidyl transferase-assisted rolling circle amplification

Chem Commun (Camb). 2018 Jan 16;54(6):682-685. doi: 10.1039/c7cc09337c.

Abstract

We proposed a terminal deoxynucleotidyl transferase (TdT)-assisted rolling circle amplification (RCA) strategy for the amplified detection of genome-containing biological targets. The synergetic signal amplification of DNase I-mediated genomic DNA fragmentation and subsequent RCA allow the sensing platform to have extraordinarily high sensitivity.

MeSH terms

  • Benzothiazoles
  • Biosensing Techniques
  • DNA / chemistry*
  • DNA / genetics
  • DNA Nucleotidylexotransferase / chemistry
  • DNA Nucleotidylexotransferase / metabolism*
  • DNA-Directed DNA Polymerase
  • Fluorescent Dyes
  • Genome
  • Nucleic Acid Amplification Techniques / methods*
  • Thiazoles / chemistry

Substances

  • Benzothiazoles
  • Fluorescent Dyes
  • Thiazoles
  • thioflavin T
  • DNA
  • DNA Nucleotidylexotransferase
  • DNA-Directed DNA Polymerase