Platelet-derived growth factor signaling modulates adult hair follicle dermal stem cell maintenance and self-renewal

Raquel González; Garrett Moffatt; Andrew Hagner; Sarthak Sinha; Wisoo Shin; Waleed Rahmani; Andrew Chojnacki; Jeff Biernaskie

doi:10.1038/s41536-017-0013-4

Platelet-derived growth factor signaling modulates adult hair follicle dermal stem cell maintenance and self-renewal

NPJ Regen Med. 2017 Apr 14:2:11. doi: 10.1038/s41536-017-0013-4. eCollection 2017.

Authors

Raquel González^#¹, Garrett Moffatt^#¹, Andrew Hagner¹, Sarthak Sinha¹, Wisoo Shin¹, Waleed Rahmani¹, Andrew Chojnacki¹, Jeff Biernaskie^{1

2

3

4}

Affiliations

¹ Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta T2N 4N1 Canada.
² Department of Surgery, Faculty of Medicine, University of Calgary, Calgary, Alberta T2N 4N1 Canada.
³ Alberta Children's Hospital Research Institute, University of Calgary, Calgary, Alberta T2N 4N1 Canada.
⁴ Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta T2N 4N1 Canada.

^# Contributed equally.

Abstract

Hair follicle regeneration is dependent on reciprocal signaling between epithelial cells and underlying mesenchymal cells within the dermal papilla. Hair follicle dermal stem cells reside within the hair follicle mesenchyme, self-renew in vivo, and function to repopulate the dermal papilla and regenerate the connective tissue sheath with each hair cycle. The identity and temporal pattern of signals that regulate hair follicle dermal stem cell function are not known. Here, we show that platelet-derived growth factor signaling is crucial for hair follicle dermal stem cell function and platelet-derived growth factor deficiency results in a progressive depletion of the hair follicle dermal stem cell pool and their progeny. Using αSMACreER^T2:Rosa^YFP:Pdgfrα^flox mice, we ablated Pdgfrα specifically within the adult hair follicle dermal stem cell lineage. This led to significant loss of hair follicle dermal stem cell progeny in connective tissue sheath and dermal papilla of individual follicles, and a progressive reduction in total number of anagen hair follicles containing YFP^+ve cells. As well, over successive hair cycles, fewer hair follicle dermal stem cells were retained within each telogen hair follicle suggesting an impact on hair follicle dermal stem cell self-renewal. To further assess this, we grew prospectively isolated hair follicle dermal stem cells (Sox2GFP^+ve αSMAdsRed^+ve) in the presence or absence of platelet-derived growth factor ligands. Platelet-derived growth factor-BB enhanced proliferation, increased the frequency of Sox2^+ve hair follicle dermal stem cell progeny and improved inductive capacity of hair follicle dermal stem cells in an ex vivo hair follicle formation assay. Similar effects on proliferation were observed in adult human SKPs. Our findings impart novel insights into the signals that comprise the adult hair follicle dermal stem cell niche and suggest that platelet-derived growth factor signaling promotes self renewal, is essential to maintain the hair follicle dermal stem cell pool and ultimately their regenerative capacity within the hair follicle.