Whole-Genome Sequencing of Six Borrelia miyamotoi Clinical Strains Isolated in Russia

Konstantin V Kuleshov; Joris Koetsveld; Irina A Goptar; Mikhail L Markelov; Nadezhda M Kolyasnikova; Denis S Sarksyan; Marina G Toporkova; Nina P Kirdyashkina; German A Shipulin; Joppe W Hovius; Alexander E Platonov

doi:10.1128/genomeA.01424-17

Whole-Genome Sequencing of Six Borrelia miyamotoi Clinical Strains Isolated in Russia

Genome Announc. 2018 Jan 4;6(1):e01424-17. doi: 10.1128/genomeA.01424-17.

Authors

Konstantin V Kuleshov^{1

2}, Joris Koetsveld³, Irina A Goptar^{4

5}, Mikhail L Markelov⁵, Nadezhda M Kolyasnikova^{4

6}, Denis S Sarksyan^{4

7}, Marina G Toporkova^{4

8}, Nina P Kirdyashkina⁵, German A Shipulin⁴, Joppe W Hovius³, Alexander E Platonov⁴

Affiliations

¹ Central Research Institute of Epidemiology, Moscow, Russia konstantinkul@gmail.com.
² Kovalenko All-Russian Research Institute for Experimental Veterinary Medicine, Moscow, Russia.
³ Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
⁴ Central Research Institute of Epidemiology, Moscow, Russia.
⁵ Research Institute of Occupational Health, Moscow, Russia.
⁶ Chumakov Federal Scientific Center for Research and Development of Immunobiological Products of Russian Academy of Sciences, Moscow, Russia.
⁷ Izhevsk State Medical Academy, Izhevsk, Russia.
⁸ Medical Association "Novaya Bolnitsa," Yekaterinburg, Russia.

Abstract

Here, we report the whole-genome sequence of six clinical Borrelia miyamotoi isolates from the Russian Federation. Using two independent next-generation sequencing platforms, we determined the complete sequence of the chromosome and several plasmids. All strains have an Asian genotype with 99.8% chromosome nucleotide similarity with B. miyamotoi strain FR64b.