Formation of Silver Nanoparticles Using Fluorescence Properties of Chitosan Oligomers

Mar Drugs. 2018 Jan 3;16(1):11. doi: 10.3390/md16010011.

Abstract

In this study, silver chloride nanoparticles (AgCl NPs) were prepared using chitosan oligomer (CHI) and chitosan oligomer derivatives (CHI-FITC). The CHI and CHI-FITC were used as markers to confirm the formation of AgCl NPs using their fluorescence properties as well as stabilizers. The fluorescence properties of CHI and CHI-FITC were monitored by a luminescence spectrophotometer, and the morphology of the AgCl NPs was further confirmed by transmission electron microscopy (TEM) and X-ray diffraction (XRD). The fluorescence of CHI and CHI-FITC was quenched by the formation of AgCl NPs, and the Stern-Volmer equation was used to compare the two types of stabilizer. The CHI and CHI-FITC stabilizer were linear and nonlinear, respectively, with respect to the Stern-Volmer equation, and considered to be usable as fluorescence indicators to confirm the formation behavior of AgCl NPs through fluorescence quenching.

Keywords: chitosan oligomer; fluorescence; nanoparticles; quenching; silver chloride (AgCl).

MeSH terms

  • Chitosan / chemistry*
  • Drug Carriers / chemistry
  • Fluorescein-5-isothiocyanate / chemistry*
  • Fluorescence
  • Metal Nanoparticles*
  • Microscopy, Electron, Transmission
  • Nanotechnology
  • Silver Compounds / chemistry*
  • X-Ray Diffraction

Substances

  • Drug Carriers
  • Silver Compounds
  • Chitosan
  • Fluorescein-5-isothiocyanate
  • silver chloride