Osteogenic differentiation of mouse bone marrow stromal cells on fibroin microcarriers

A V Goncharenko; M S Kotlyarova; A M Moisenovich; A Y Arkhipova; D A Kulikov; A S Konkov; A V Kulikov; A E Mashkov; I I Agapov; M M Moisenovich; M P Kirpichnikov

doi:10.1134/S1607672917060011

Osteogenic differentiation of mouse bone marrow stromal cells on fibroin microcarriers

Dokl Biochem Biophys. 2017 Nov;477(1):345-348. doi: 10.1134/S1607672917060011. Epub 2018 Jan 3.

Authors

Affiliations

¹ Biological Faculty, Moscow State University, Moscow, 119992, Russia.
² Vladimirskii Moscow Regional Research Clinical Institute, ul. Shchepkina 61/2, Moscow, 129110, Russia.
³ Institute of Theoretical and Experimental Biophysics, Pushchino Branch, Russian Academy of Sciences, Pushchino, Moscow oblast, 142292, Russia.
⁴ Shumakov Research Institute of Transplantation and Artificial Organs, Russian Ministry of Health, Moscow, 113182, Russia.
⁵ Biological Faculty, Moscow State University, Moscow, 119992, Russia. mmoisenovich@mail.ru.

PMID: 29297117
DOI: 10.1134/S1607672917060011

Abstract

We investigated the proliferation and osteogenic differentiation of mesenchymal stem cells cultured on fibroin microcarriers. Effective cell proliferation on the surface of the microcarriers, determined by the large surface area, and the contribution of microcarrier mineralization to the stimulation of the osteogenic differentiation of mesenchymal stem cells was revealed.

MeSH terms

Animals
Bone Marrow Cells / cytology*
Cell Culture Techniques / methods*
Cell Differentiation
Cell Proliferation
Fibroins / chemistry*
Gelatin / chemistry
Indoles / chemistry
Mesenchymal Stem Cells / cytology*
Mice
Microspheres*
Osteogenesis*

Substances

Indoles
DAPI
Gelatin
Fibroins