Abstract
The development of metabolic oligosaccharide engineering (MOE) over the past two decades enabled the bioimaging studies of glycosylation processes in physio-pathological contexts. Herein, we successfully applied the chemical reporter strategy to image the fate of sialylated glycoconjugates in healthy and sialin-deficient patient fibroblasts. This chemical glycomics enrichment is a powerful tool for tracking sialylated glycoconjugates and probing lysosomal recycling capacities. Thus, such strategies appear fundamental for the characterization of lysosomal storage diseases.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Case-Control Studies
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Chemical Fractionation
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Combinatorial Chemistry Techniques / methods
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Glycomics / methods*
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Humans
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Lysosomal Storage Diseases / diagnosis
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Lysosomal Storage Diseases / metabolism
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Lysosomes / metabolism
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Metabolic Engineering / methods*
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Metabolic Networks and Pathways / physiology
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N-Acetylneuraminic Acid / analysis*
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N-Acetylneuraminic Acid / metabolism*
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Oligosaccharides / analysis
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Oligosaccharides / chemistry
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Oligosaccharides / metabolism*
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Single Molecule Imaging / methods*
Substances
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Oligosaccharides
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N-Acetylneuraminic Acid