Chemical glycomics enrichment: imaging the recycling of sialic acid in living cells

J Inherit Metab Dis. 2018 May;41(3):515-523. doi: 10.1007/s10545-017-0118-3. Epub 2018 Jan 2.

Abstract

The development of metabolic oligosaccharide engineering (MOE) over the past two decades enabled the bioimaging studies of glycosylation processes in physio-pathological contexts. Herein, we successfully applied the chemical reporter strategy to image the fate of sialylated glycoconjugates in healthy and sialin-deficient patient fibroblasts. This chemical glycomics enrichment is a powerful tool for tracking sialylated glycoconjugates and probing lysosomal recycling capacities. Thus, such strategies appear fundamental for the characterization of lysosomal storage diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Case-Control Studies
  • Chemical Fractionation
  • Combinatorial Chemistry Techniques / methods
  • Glycomics / methods*
  • Humans
  • Lysosomal Storage Diseases / diagnosis
  • Lysosomal Storage Diseases / metabolism
  • Lysosomes / metabolism
  • Metabolic Engineering / methods*
  • Metabolic Networks and Pathways / physiology
  • N-Acetylneuraminic Acid / analysis*
  • N-Acetylneuraminic Acid / metabolism*
  • Oligosaccharides / analysis
  • Oligosaccharides / chemistry
  • Oligosaccharides / metabolism*
  • Single Molecule Imaging / methods*

Substances

  • Oligosaccharides
  • N-Acetylneuraminic Acid