Abstract
FlAlyA is an endolytic enzyme with a preference for polymannuronate. The crystal structure and mutagenesis studies elucidated that the structural variations at outer uronate-binding subsites +2, +3 and -2 control the enzymatic properties of PL-7 family enzymes. Lys158 mutations changed the pH dependency and enhanced the production of mono- and disaccharides.
MeSH terms
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Alginates / chemistry
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Alginates / metabolism*
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Flavobacterium / enzymology
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Glucuronic Acid / chemistry
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Glucuronic Acid / metabolism
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Hexuronic Acids / chemistry
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Hexuronic Acids / metabolism
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Hydrogen-Ion Concentration
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Models, Molecular
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Molecular Structure
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Polysaccharide-Lyases / chemistry
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Polysaccharide-Lyases / genetics
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Polysaccharide-Lyases / metabolism*
Substances
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Alginates
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Hexuronic Acids
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Glucuronic Acid
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Polysaccharide-Lyases
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poly(beta-D-mannuronate) lyase