Rapid detection of clenbuterol in milk using microfluidic paper-based ELISA

Luyao Ma; Azadeh Nilghaz; Jane Ru Choi; Xiaoqing Liu; Xiaonan Lu

doi:10.1016/j.foodchem.2017.12.022

Rapid detection of clenbuterol in milk using microfluidic paper-based ELISA

Food Chem. 2018 Apr 25:246:437-441. doi: 10.1016/j.foodchem.2017.12.022. Epub 2017 Dec 7.

Authors

Luyao Ma¹, Azadeh Nilghaz¹, Jane Ru Choi¹, Xiaoqing Liu¹, Xiaonan Lu²

Affiliations

¹ Food, Nutrition and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, BC V6T1Z4, Canada.
² Food, Nutrition and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, BC V6T1Z4, Canada. Electronic address: xiaonan.lu@ubc.ca.

PMID: 29291870
DOI: 10.1016/j.foodchem.2017.12.022

Abstract

In this study, a paper-based microfluidic enzyme-linked immunosorbent assay (ELISA) was developed as a screening system for rapid detection of clenbuterol, which is illegally used as a growth promoter for food-producing animals. The microfluidic paper-based analytical device (μPAD) was combined with ELISA and the intrinsic properties of paper allowed the entrapment of antibody through cellulosic fibres, validating to be an alternative to 96-well ELISA microplate for food safety monitoring. Detection of clenbuterol in milk was achieved by measuring the intensity of colour change that was proportional to the analyte concentration with a detection limit of 0.2 ppb. The μPAD effectively reduces the cost, volume of reagents, and time required to run ELISA for food sample testing.

Keywords: Clenbuterol; ELISA; Food safety; Immunoassay; Milk; Paper-based microfluidics.

MeSH terms

Animals
Clenbuterol / analysis*
Enzyme-Linked Immunosorbent Assay / instrumentation*
Enzyme-Linked Immunosorbent Assay / methods
Food Contamination / analysis*
Lab-On-A-Chip Devices*
Limit of Detection
Microfluidic Analytical Techniques / instrumentation
Milk / chemistry*
Paper

Substances

Clenbuterol