Mobile phone based ELISA (MELISA)

Arsenii Zhdanov; Jordan Keefe; Luis Franco-Waite; Karthik Raj Konnaiyan; Anna Pyayt

doi:10.1016/j.bios.2017.12.033

Mobile phone based ELISA (MELISA)

Biosens Bioelectron. 2018 Apr 30:103:138-142. doi: 10.1016/j.bios.2017.12.033. Epub 2017 Dec 21.

Authors

Arsenii Zhdanov¹, Jordan Keefe¹, Luis Franco-Waite¹, Karthik Raj Konnaiyan¹, Anna Pyayt²

Affiliations

¹ Department of Chemical and Biomedical Engineering, University of South Florida, Tampa, USA.
² Department of Chemical and Biomedical Engineering, University of South Florida, Tampa, USA. Electronic address: pyayt@usf.edu.

PMID: 29291593
DOI: 10.1016/j.bios.2017.12.033

Abstract

Enzyme-linked immunosorbent assay (ELISA) is one of the most important technologies for biochemical analysis critical for diagnosis and monitoring of many diseases. Traditional systems for ELISA incubation and reading are expensive and bulky, thus cannot be used at point-of-care or in the field. Here, we propose and demonstrate a new miniature mobile phone based system for ELISA (MELISA). This system can be used to complete all steps of the assay, including incubation and reading. It weighs just 1 pound, can be fabricated at low cost, portable, and can transfer test results via mobile phone. We successfully demonstrated how MELISA can be calibrated for accurate measurements of progesterone and demonstrated successful measurements with the calibrated system.

Keywords: Enzyme-linked immunosorbent assay (ELISA); Mobile ELISA (MELISA); Optical sensor; Progesterone.

MeSH terms

Biosensing Techniques / methods*
Cell Phone*
Enzyme-Linked Immunosorbent Assay / methods*
Humans
Point-of-Care Systems