Conductive nanofibrous composite scaffolds based on in-situ formed polyaniline nanoparticle and polylactide for bone regeneration

Jing Chen; Meng Yu; Baolin Guo; Peter X Ma; Zhanhai Yin

doi:10.1016/j.jcis.2017.12.062

Conductive nanofibrous composite scaffolds based on in-situ formed polyaniline nanoparticle and polylactide for bone regeneration

J Colloid Interface Sci. 2018 Mar 15:514:517-527. doi: 10.1016/j.jcis.2017.12.062. Epub 2017 Dec 24.

Authors

Jing Chen¹, Meng Yu², Baolin Guo³, Peter X Ma⁴, Zhanhai Yin⁵

Affiliations

¹ Xi'an Modern Chemistry Research Institute, Xi'an 710065, China; Frontier Institute of Science and Technology, State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
² Frontier Institute of Science and Technology, State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
³ Frontier Institute of Science and Technology, State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China. Electronic address: baoling@mail.xjtu.edu.cn.
⁴ Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, USA; Macromolecular Science and Engineering Center, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: mapx@umich.edu.
⁵ Department of Orthopaedics, The First Affiliated Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710061, China. Electronic address: zhanhai.yin@mail.xjtu.edu.cn.

PMID: 29289734
DOI: 10.1016/j.jcis.2017.12.062

Abstract

Conducting polymers and biodegradable polylactide (PLA) scaffolds are both promising biomaterials applied in bone tissue engineering. It is necessary to develop a composite scaffold combining their properties of osteogenic differentiation promotion and three-dimension matrix. To conquer the problem of poor processability of conductive polymers, we use a novel in-situ polymerization/thermal induced phase separation (TIPS) method to fabricate conductive nanofibrous PLA scaffolds with well-distributed polyaniline (PANI) nano-structures. The simple preparation technique provides the possibility to scale-up production of these conductive nanofibrous composite scaffolds. The scaffold structure and content of in-situ formed polyaniline nanoparticles was thoroughly characterized with ¹H NMR, FT-IR, XPS, TGA, SEM and UV-vis, and the conductivity/electrochemical properties of the composite scaffolds were controlled with varied feed ratios of aniline to PLA. Meanwhile, the good cytocompatibility of these composite scaffolds was evaluated by culturing bone marrow derived mesenchymal stem cells (BMSCs) on them. The effect of conductive nanofibrous scaffolds on osteogenic differentiation was studied with expression levels of alkaline phosphatase (Alp), osteocalcin (Ocn) and runt-related transcription factor 2 (Runx2) during the culture of BMSCs for three weeks. The calcium mineralization of BMSCs is determined by alizarin red staining. These results indicated that a moderate content of PANI in the conductive nanofibrous scaffolds significantly promoted osteogenic differentiation of BMSCs for engineering bone tissues.

Keywords: Composite nanofibrous scaffold; Conductive polymer; Osteogenic differentiation; Polyaniline nanoparticle; Polylactide.

MeSH terms

Aniline Compounds / chemistry
Aniline Compounds / pharmacology*
Bone Marrow / drug effects*
Bone Regeneration / drug effects*
Cell Differentiation / drug effects
Cell Proliferation / drug effects
Cell Survival / drug effects
Cells, Cultured
Electric Conductivity
Humans
Mesenchymal Stem Cells / drug effects*
Nanofibers / chemistry*
Osteoblasts / drug effects
Particle Size
Polyesters / chemistry
Polyesters / pharmacology*
Surface Properties

Substances

Aniline Compounds
Polyesters
polyaniline
poly(lactide)