A New, Nondestructive, Split-Root System for Local and Systemic Plant Responses Studies with Soybean

Ángeles Hidalgo; José E Ruiz-Sainz; José M Vinardell

doi:10.1007/978-1-4939-7604-1_23

A New, Nondestructive, Split-Root System for Local and Systemic Plant Responses Studies with Soybean

Methods Mol Biol. 2018:1734:297-306. doi: 10.1007/978-1-4939-7604-1_23.

Authors

Ángeles Hidalgo¹, José E Ruiz-Sainz¹, José M Vinardell²

Affiliations

¹ Department of Microbiology, Faculty of Biology, University of Seville, Avda. Reina Mercedes 6, 41012, Seville, Spain.
² Department of Microbiology, Faculty of Biology, University of Seville, Avda. Reina Mercedes 6, 41012, Seville, Spain. jvinar@us.es.

PMID: 29288463
DOI: 10.1007/978-1-4939-7604-1_23

Abstract

Plants use long-distance signaling mechanisms to coordinate their growth and control their interactions, positive or negative, with microbes. Split-root systems (SRS) have been used to study the relevance of both local and systemic plant mechanisms that participate in the control of rhizobia-legume symbioses. In this work we have developed a modification of the standard split-root system (SRS) used with soybean. This modified method, unlike previous systems, operates in hydroponics conditions and therefore is nondestructive and allows for the continuous monitoring of soybean roots throughout the whole experiment.

Keywords: Autoregulation of nodulation; Hydroponic culture; Nodulation; Soybean; Split-root system.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Germination
Glycine max / physiology*
Plant Root Nodulation / physiology*
Plant Roots / physiology*
Seedlings
Seeds / physiology