Airway microbial metagenomics

Katarzyna Pienkowska; Lutz Wiehlmann; Burkhard Tümmler

doi:10.1016/j.micinf.2017.12.002

Airway microbial metagenomics

Microbes Infect. 2018 Oct-Nov;20(9-10):536-542. doi: 10.1016/j.micinf.2017.12.002. Epub 2017 Dec 26.

Authors

Katarzyna Pienkowska¹, Lutz Wiehlmann², Burkhard Tümmler³

Affiliations

¹ Clinical Research Group, Clinic for Pediatric Pneumology, Allergology, and Neonatology, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.
² Clinical Research Group, Clinic for Pediatric Pneumology, Allergology, and Neonatology, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany; Core Unit 'Genomics', Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.
³ Clinical Research Group, Clinic for Pediatric Pneumology, Allergology, and Neonatology, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany; Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), German Center for Lung Research, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany. Electronic address: tuemmler.burkhard@mh-hannover.de.

PMID: 29287982
DOI: 10.1016/j.micinf.2017.12.002

Abstract

High-throughput untargeted metagenome sequencing provides information about the composition of the microbial communities of viruses, bacteria, archaea and unicellular eukaryotes in the habitat of interest. This review outlines the sampling, processing, sequencing and bioinformatic analysis of secretions of the respiratory tract and summarizes our current knowledge of the upper and lower human airways metagenome in health and disease.

Keywords: Chronic obstructive pulmonary disease; Cystic fibrosis; Metagenome; Respiratory tract.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Bacteria / classification
Bacteria / genetics
Bacteria / isolation & purification
Computational Biology
High-Throughput Nucleotide Sequencing
Humans
Lung Diseases / microbiology*
Metagenomics*
Microbiota / genetics*
Respiratory System / microbiology*
Viruses / classification
Viruses / genetics
Viruses / isolation & purification