Conformation stability, in vitro digestibility and allergenicity of tropomyosin from shrimp (Exopalaemon modestus) as affected by high intensity ultrasound

Ziye Zhang; Xiaofeng Zhang; Wei Chen; Peng Zhou

doi:10.1016/j.foodchem.2017.11.072

Conformation stability, in vitro digestibility and allergenicity of tropomyosin from shrimp (Exopalaemon modestus) as affected by high intensity ultrasound

Food Chem. 2018 Apr 15:245:997-1009. doi: 10.1016/j.foodchem.2017.11.072. Epub 2017 Nov 21.

Authors

Ziye Zhang¹, Xiaofeng Zhang², Wei Chen³, Peng Zhou⁴

Affiliations

¹ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province 214122, China.
² Department of Clinical Laboratory, Wuxi People's Hospital, Wuxi, Jiangsu 214023, China.
³ Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu Province 214122, China.
⁴ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province 214122, China. Electronic address: zhoupeng@jiangnan.edu.cn.

PMID: 29287471
DOI: 10.1016/j.foodchem.2017.11.072

Abstract

The effects of high intensity ultrasound (HIU) modification (power: 100-800 W, 15 min) on the conformation stability, in vitro digestibility and allergenicity of tropomyosin (TM) from Exopalaemon modestus were investigated. HIU treatment oxidized the Cys, Met and Lys in TM and degraded TM to generate protein fragments by HIU-induced free radicals. The α-helix content decreased, while β-sheet, β-turn and random coil increased. The immunoblotting and ELISA data suggested HIU treatment reduced the allergenicity and contributed to higher digestibility of TM. TM had resistance to gastric digestion, while intestinal digestion totally hydrolyzed TM, and the sequential HIU-gastric-intestinal digestion most efficiently hydrolyzed TM and reduced the allergenicity of digestion products. Stronger HIU could loosen TM structure, strengthen the gastrointestinal digestibility of TM and reduce the allergenicity of TM and digestion products to low level.

Keywords: Allergenicity; Conformation; Digestibility; Exopalaemon modestus; High intensity ultrasound; Tropomyosin.

MeSH terms

Animals
Digestion*
Palaemonidae / chemistry*
Protein Conformation, alpha-Helical
Protein Conformation, beta-Strand
Protein Stability
Seafood / analysis
Tropomyosin / chemistry*
Tropomyosin / immunology*
Tropomyosin / metabolism
Ultrasonic Waves*

Substances

Tropomyosin