Objective: to investigate morphological changes in the primary thyroid cell culture of rat infants whose parents were prenatally exposed by radioisotope iodine 131.
Materials and methods: obtaining and culturing of thyroid tissue primary cell cultures of newborn rats, cytological (receipt and analysis of cell cultures agents for optical microscopy), biophysical (flow cytometry), statistics.
Results: It was shown that cells in thyroid primary culture of offspring rats prenatally exposed by radioisotopes of iodine 131 signs of destructive degenerative changes were observed mostly when animals of both sexes were irra diated. Increased number of two and three nuclear cells and induction of ring like cells is an evidence of signifi cant genotoxic violation and points to the genome instability in offspring of animals exposed by radioisotope iodine 131.
Conclusions: Analysis and quantitative morphological parameters of cells in thyroid primary culture of newborn rats whose parents were exposed prenatally by radioisotopes of iodine 131 showed that upon exposure to radiation thy roid undergoes destructive changes at the cellular level and, even in the second generation of offspring, leads to disruption of its functions.
Meta: doslidyty morfologichni zminy v pervynniy̆ kul'turi klityn shchytopodibnoï zalozy novonarodzhenykh shchuriv, bat'ky iakykh buly prenatal'no opromineni radioizotopom y̆odu 131.Materialy i metody: otrymannia ta kul'tyvuvannia pervynnykh kul'tur klityn iz tkanyn shchytopodibnoï zalozy no vonarodzhenykh shchuriv, tsytologichni (otrymannia ta analiz preparativ kul'tur klityn dlia optychnoï mikroskopiï), biofizychni (protokova tsytofliuorymetriia), statystychni.Rezul'taty: bulo pokazano, shcho v pervynniy̆ kul'turi klityn shchytopodibnoï zalozy nashchadkiv shchuriv, prenatal'no oprominenykh radioizotopamy y̆odu 131, sposterigaly oznaky destruktyvno degeneratsiy̆nykh zmin, iaki buly nay̆bil'sh vyrazheni pry oprominenni tvaryn obokh statey̆. Zbil'shennia kil'kosti dvo i tryiadernykh klityn ta induktsiia persnepodibnykh klityn svidchat' pro istotni genotoksychni porushennia i vkazuiut' na nestabil'nist' geno mu u nashchadkiv tvaryn, oprominenykh radioizotopom y̆odu 131.Vysnovky. Analiz ta kil'kisna kharakterystyka morfofunktsional'nykh pokaznykiv u pervynniy̆ kul'turi klityn shchytopodibnoï zalozy novonarodzhenykh shchuriv, bat'ky iakykh buly opromineni prenatal'no radioizotopamy y̆odu 131, pokazala, shcho pid vplyvom oprominennia shchytopodibna zaloza zaznaie destruktyvnykh zmin na klitynnomu rivni navit' u nashchadkiv drugogo pokolinnia i pryzvodyt' do porushennia ïï funktsiy̆.
Keywords: apoptosis; mitosis; proliferation; radioisotope iodine 131; thyroid cell culture of rat.
O. A. Boiko, H. Yo. Lavrenchuk, A. I. Lypska, V. V. Talko, V. S. Asmolkov.