CD14-159C/T polymorphism in the development of delayed skin hypersensitivity to tuberculin

Magdalena Druszczynska; Marcin Wlodarczyk; Grzegorz Kielnierowski; Michal Seweryn; Sebastian Wawrocki; Wieslawa Rudnicka

doi:10.1371/journal.pone.0190106

CD14-159C/T polymorphism in the development of delayed skin hypersensitivity to tuberculin

PLoS One. 2017 Dec 27;12(12):e0190106. doi: 10.1371/journal.pone.0190106. eCollection 2017.

Authors

Magdalena Druszczynska¹, Marcin Wlodarczyk¹, Grzegorz Kielnierowski², Michal Seweryn³, Sebastian Wawrocki¹, Wieslawa Rudnicka¹

Affiliations

¹ Division of Cell Immunology, Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
² Regional Specialized Hospital of Tuberculosis, Lung Diseases and Rehabilitation, Tuszyn, Poland.
³ Center for Medical Genomics OMICRON, Jagiellonian University, Medical College, Cracow, Poland.

Abstract

The skin tuberculin test (TST), an example of a delayed-type hypersensitivity (DTH) reaction, is based on measuring the extent of skin induration to mycobacterial tuberculin (PPD). Little is known about the genetic basis of TST reactivity, widely used for diagnosing TB infection. The study investigated the relationship of the single base change polymorphic variants in CD14 gene (CD14(-159C/T)) with the development of DTH to PPD in BCG-vaccinated Polish Caucasian individuals. We found persistent lack of TST reactivity in about 40% of healthy subjects despite receiving more than one dose of BCG. The TST size was negatively correlated with the number of BCG inoculations. The distribution of C/T genotype was significantly more frequent among TST-negative compared with TST-positive individuals. The concentration of serum sCD14 was positively associated with mCD14 expression, but not with the TST status or CD14(-159C/T) polymorphism. A significant increase in mCD14 expression and serum sCD14 levels was found in TB group. We hypothesize that CD14(-159C/T) polymorphic variants might be one of genetic components in the response to attenuated M. bovis BCG bacilli.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Female
Humans
Hypersensitivity, Delayed*
Lipopolysaccharide Receptors / immunology*
Male
Polymorphism, Genetic*
Tuberculin / adverse effects*
Tuberculin Test
Young Adult

Substances

Lipopolysaccharide Receptors
Tuberculin

Grants and funding

This work was supported by the grant from the Polish Ministry of Science and Higher Education (N N402 098539). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.