Overview of the improvement of the ring-stage survival assay-a novel phenotypic assay for the detection of artemisinin-resistant Plasmodium falciparum

Zool Res. 2017 Nov 18;38(6):317-320. doi: 10.24272/j.issn.2095-8137.2017.075.

Abstract

Artemisinin resistance in Plasmodium falciparum threatens the remarkable efficacy of artemisinin-based combination therapies worldwide. Thus, greater insight into the resistance mechanism using monitoring tools is essential. The ring-stage survival assay is used for phenotyping artemisinin-resistance or decreased artemisinin sensitivity. Here, we review the progress of this measurement assay and explore its limitations and potential applications.

Keywords: Application; Artemisinin; Improvement; Malaria; Resistant phenotype; Ring-stage survival assay.

Publication types

  • Review

MeSH terms

  • Antimalarials / pharmacology*
  • Artemisinins / pharmacology*
  • Biological Assay / methods
  • Drug Resistance*
  • Humans
  • Malaria, Falciparum / parasitology
  • Plasmodium falciparum / drug effects*
  • Plasmodium falciparum / isolation & purification

Substances

  • Antimalarials
  • Artemisinins
  • artemisinin

Grants and funding

This study was funded by the National Natural Science Foundation of China (31260508, U1202226) and grants of Doctor Newcomer Scholarship from Yunnan province