The transcriptional factor YB-1 positively regulates Hsc70 transcription in Crassostrea hongkongensis

Huiru Liu; Delin Xu; Miao Cui; Tinglong Hou; Qizhong Zhang

doi:10.1016/j.bbrc.2017.12.110

The transcriptional factor YB-1 positively regulates Hsc70 transcription in Crassostrea hongkongensis

Biochem Biophys Res Commun. 2018 Jan 22;495(4):2404-2409. doi: 10.1016/j.bbrc.2017.12.110. Epub 2017 Dec 23.

Authors

Huiru Liu¹, Delin Xu¹, Miao Cui¹, Tinglong Hou¹, Qizhong Zhang²

Affiliations

¹ Department of Ecology, Institute of Hydrobiology, School of Life Science and Technology, Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Ministry of Education, Jinan University, Guangzhou, 510632, PR China.
² Department of Ecology, Institute of Hydrobiology, School of Life Science and Technology, Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Ministry of Education, Jinan University, Guangzhou, 510632, PR China. Electronic address: zhangqzdr@126.com.

PMID: 29277610
DOI: 10.1016/j.bbrc.2017.12.110

Abstract

A Y-box binding protein ChYB-1 was discovered as a ChHsc70 promoter-associated protein in Crassostrea hongkongensis by DNA-affinity purification and mass spectrometry analysis. The overexpression of ChYB-1 in heterologous HEK293T cells led to clear enhancement of ChHsc70 promoter expression, while ChYB-1 depletion correlated with significant reduction of ChHsc70 transcription in the hemocytes of C. hongkongensis. Quantitative Real-time PCR analysis revealed that both ChHsc70 and ChYB-1 were transcriptionally responsive to external chemical or physical stressors. This indicates a plausible correlation between ChHsc70 and ChYB-1 in the genetic regulatory pathway triggered by external stresses. This study presents the first evidence of positive regulator for Hsc70 transcription and contributes to a better understanding of the regulatory mechanisms governing Hsc70 expression.

Keywords: Crassostrea hongkongensis; Hsc70; Transcriptional regulator; YB-1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Crassostrea / metabolism*
Gene Expression Regulation / physiology*
HSC70 Heat-Shock Proteins / metabolism*
Oxidative Stress / physiology
Transcription Factors / metabolism*
Transcriptional Activation / physiology*
Y-Box-Binding Protein 1 / metabolism*

Substances

HSC70 Heat-Shock Proteins
Transcription Factors
Y-Box-Binding Protein 1