Objective: To evaluate gray matter structure changes in long-term male smokers by voxel-based morphological method. Methods: Fifty long-term smokers and 37 non-smoking healthy volunteers were scanned with Siemens Skyro 3.0T magnetic resonance scanner from August 2014 to August 2016. The subjects underwent routine MRI (excluding intracranial lesions) sequences and 3D-T1 structural sequences (3D-mprage). SPM8 pretreatment based on Matlab was used to analyze the structural data. All of the data were analyzed by SPM8 software. The data were compared between groups with independent sample t test. Spearman correlation analysis was used to analyze the relationship between gray matter volume (GMV) and smoking data of two groups. Results: The gray matter volume of bilateral thalamic, right supramarginal gyrus, left supramarginal gyrus and left putamen of smoking group were (0.55±0.07), (0.40±0.05), (0.48±0.07) and (0.14±0.04) voxels, respectively, and the gray matter volume of the corresponding gyri in control group were (0.61±0.09), (0.43±0.06), (0.54±0.07) and (0.16±0.03) voxels, respectively; and the gray matter volume of smoking group were all lower than those in control group (t=-3.81, -3.51, -3.86, -2.33, all P<0.05), family wise error (FWE) correction (P<0.05). The gray matter volume of bilateral thalamus, right supramarginal gyrus and left putamen was negatively correlated with smoking index (r=-0.368, -0.189, -0.274, all P<0.05), and also negatively correlated with smoking years (r=-0.391, -0.221, -0.355, all P<0.05), and bilateral thalamus gray matter volume was negatively correlated with daily cigarette smoking (r=-0.186, P<0.05). Conclusion: The changes of brain structure of smokers mainly occur on reward-related pathways and marginal systems, and related to accumulation of cigarette smoking.
目的： 应用基于体素的形态学分析(VBM)方法评价长期吸烟对男性吸烟者脑灰质结构改变的影响。 方法： 2014年8月至2016年8月采用西门子Skyro 3.0T MRI扫描仪对50名长期吸烟者和37名不吸烟的健康志愿者进行扫描，受试者均进行常规MRI(以排除颅内病变)序列及3D－T1结构像(3D－mprage)序列扫描。结构像数据采用基于Matlab平台的SPM8预处理，统计学分析采用SPM8软件，对两组资料进行独立样本t检验，将两组的灰质差异区和吸烟资料做Spearman相关分析。 结果： 吸烟组双侧丘脑、右侧缘上回、左侧缘上回和左侧壳核灰质体积分别为(0.55±0.07)、(0.40±0.05)、(0.48±0.07)和(0.14±0.04)体素，对照组相应脑区灰质体积分别为(0.61±0.09)、(0.43±0.06)、(0.54±0.07)和(0.16±0.03)体素，吸烟组均低于对照组(t＝－3.81、－3.51、－3.86、－2.33，均P<0.05)，族系误差校正(P<0.05)。双侧丘脑、右侧缘上回、左侧壳核灰质体积与吸烟指数呈负相关(r＝－0.368、－0.189、－0.274，均P<0.05)，且与吸烟年限也呈负相关(r＝－0.391、－0.221、－0.355，均P<0.05)，双侧丘脑灰质体积还与日吸烟量呈负相关(r＝－0.186，P<0.05)。 结论： 吸烟者脑灰质结构的改变主要集中在奖励相关通路及边缘系统，且与吸烟的累积量部分相关。.
Keywords: Brain; Gray matter; Magnetic resonance imaging; Men; Smoking.