Molecular tools for pathway engineering in Saccharomyces cerevisiae

Pamela B Besada-Lombana; Tami L McTaggart; Nancy A Da Silva

doi:10.1016/j.copbio.2017.12.002

Molecular tools for pathway engineering in Saccharomyces cerevisiae

Curr Opin Biotechnol. 2018 Oct:53:39-49. doi: 10.1016/j.copbio.2017.12.002. Epub 2017 Dec 20.

Authors

Pamela B Besada-Lombana¹, Tami L McTaggart¹, Nancy A Da Silva²

Affiliations

¹ Department of Chemical Engineering and Materials Science, University of California, Irvine, CA 92697-2575, USA.
² Department of Chemical Engineering and Materials Science, University of California, Irvine, CA 92697-2575, USA. Electronic address: ndasilva@uci.edu.

PMID: 29274630
DOI: 10.1016/j.copbio.2017.12.002

Abstract

Molecular tools for the regulation of protein expression in Saccharomyces cerevisiae have contributed to rapid advances in pathway engineering for this yeast. This review considers new and enhanced additions to this toolbox, focusing on experimental approaches to modulate enzyme synthesis and enzyme fate. Methods for genome engineering, regulation of transcription, post-translational protein localization, and combinatorial screening and sensing in S. cerevisiae are highlighted, and promising new approaches are introduced.

Publication types

Review

MeSH terms

Genetic Engineering
Metabolic Engineering / methods*
Promoter Regions, Genetic / genetics
Recombinant Proteins / metabolism
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism*
Transcription, Genetic

Substances

Recombinant Proteins