A simple and rapid approach to develop recombinant avian herpesvirus vectored vaccines using CRISPR/Cas9 system

Vaccine. 2018 Jan 29;36(5):716-722. doi: 10.1016/j.vaccine.2017.12.025. Epub 2018 Jan 2.

Abstract

Herpesvirus of turkeys (HVT) has been successfully used as live vaccine against Marek's disease (MD) worldwide for more than 40 years either alone or in combination with other serotypes. HVT is also widely used as a vector platform for generation of recombinant vaccines against a number of avian diseases such as infectious bursal disease (IBD), Newcastle disease (ND) and avian influenza (AI) using conventional recombination methods or recombineering tools on cloned viral genomes. In the present study, we describe the application of CRISPR/Cas9-based genome editing as a rapid and efficient method of generating HVT recombinants expressing VP2 protein of IBDV. This approach offers an efficient method to introduce other viral antigens into the HVT genome for rapid development of recombinant vaccines.

Keywords: CRISPR/Cas9; Cre-loxP; HVT; Recombinant vaccine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CRISPR-Cas Systems*
  • Chick Embryo
  • Chickens
  • Fibroblasts
  • Gene Editing
  • Gene Expression
  • Gene Knock-In Techniques
  • Gene Order
  • Gene Targeting
  • Genes, Reporter
  • Genetic Engineering
  • Genetic Vectors / genetics*
  • Herpesvirus 1, Meleagrid / genetics*
  • Mice
  • Vaccines, Synthetic / genetics*
  • Vaccines, Synthetic / immunology*
  • Virus Replication

Substances

  • Vaccines, Synthetic