A novel fluorescein-based "turn-on" probe for the detection of hydrazine and its application in living cells

Spectrochim Acta A Mol Biomol Spectrosc. 2018 Mar 15:193:324-329. doi: 10.1016/j.saa.2017.12.040. Epub 2017 Dec 14.

Abstract

We constructed a novel probe for hydrazine detection based on ICT and PET mechanism. Phthalimide and acetyl ester groups were used as the recognition units. Addition of hydrazine produced a turn-on fluorescence at 525nm along with the fluorescent color change from dark to yellow. The probe could selectively detect hydrazine over other related interfering species. The detection limit of the probe for hydrazine was calculated to be 0.057μM which was lower than the EPA standard (0.320μM). Furthermore, the probe could also be applied for the imaging of hydrazine in living cells.

Keywords: Fluorescein; Fluorescent probe; Hydrazine; Living cells; Turn-on.

MeSH terms

  • Breast Neoplasms / metabolism*
  • Female
  • Fluorescein / chemistry*
  • Fluorescent Dyes / chemistry*
  • Humans
  • Hydrazines / analysis*
  • Limit of Detection
  • Molecular Imaging / methods*
  • Spectrometry, Fluorescence
  • Tumor Cells, Cultured

Substances

  • Fluorescent Dyes
  • Hydrazines
  • hydrazine
  • Fluorescein