QTL mapping for benzoxazinoid content, preharvest sprouting, α-amylase activity, and leaf rust resistance in rye (Secale cereale L.)

PLoS One. 2017 Dec 21;12(12):e0189912. doi: 10.1371/journal.pone.0189912. eCollection 2017.

Abstract

Mapping population of recombinant inbred lines (RILs) representing 541 × Ot1-3 cross exhibited wide variations of benzoxazinoid (BX) content in leaves and roots, brown rust resistance, α-amylase activity in the grain, and resistance to preharvest sprouting. QTL mapping of major BX species using a DArT-based map revealed a complex genetic architecture underlying the production of these main secondary metabolites engaged in stress and allelopathy responses. The synthesis of BX in leaves and roots was found to be regulated by different QTL. The QTL for the BX content, rust resistance, α-amylase activity, and preharvest sprouting partially overlapped; this points to their common genetic regulation by a definite subset of genes. Only one QTL for BX located on chromosome 7R coincided with the loci of the ScBx genes, which were mapped as two clusters on chromosomes 5RS (Bx3-Bx5) and 7R (Bx1-Bx2). The QTL common for several BX species, rust resistance, preharvest sprouting, and α-amylase activity are interesting objects for further exploration aimed at developing common markers for these important agronomic traits.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Basidiomycota / pathogenicity*
  • Benzoxazines / metabolism*
  • Plant Leaves / microbiology*
  • Quantitative Trait Loci*
  • Secale / microbiology*
  • alpha-Amylases / metabolism*

Substances

  • Benzoxazines
  • alpha-Amylases

Grants and funding

This work was financially supported by the National Centre for Research and Development, Project No. PBS1/A8/12/2012 (http://www.ncbr.gov.pl/programy-krajowe/program-badan-stosowanych/). Danko Plant Breeders LTD provided partial funding for this project as a commercial partner. Danko Plant Breeders LTD also provided support in the form of salaries for Zofia Banaszak and Katarzyna Banaszak, but had no further role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the 'author contributions' section.