Characterization of KPC-Encoding Plasmids from Enterobacteriaceae Isolated in a Czech Hospital

Antimicrob Agents Chemother. 2018 Feb 23;62(3):e02152-17. doi: 10.1128/AAC.02152-17. Print 2018 Mar.

Abstract

Ten Enterobacteriaceae isolates collected in a Czech hospital carried blaKPC-positive plasmids of different sizes (∼30, ∼45, and ∼80 kb). Sequencing revealed three types of plasmids (A to C) with the Tn4401a transposon. Type A plasmids comprised an IncR backbone and a KPC-2-encoding multidrug resistance (MDR) region. Type B plasmids were derivatives of type A plasmids carrying an IncN3-like segment, while type C plasmids were IncP6 plasmids sharing the same KPC-2-encoding MDR region with type A and B plasmids.

Keywords: Citrobacter freundii; Illumina sequencing; IncR; ST18; Tn4401a.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / therapeutic use
  • Base Sequence
  • Carbapenems / therapeutic use
  • Citrobacter freundii / drug effects
  • Citrobacter freundii / enzymology
  • Citrobacter freundii / genetics*
  • Citrobacter freundii / isolation & purification
  • Czech Republic / epidemiology
  • DNA Transposable Elements
  • Drug Resistance, Multiple, Bacterial / genetics
  • Enterobacteriaceae Infections / drug therapy
  • Enterobacteriaceae Infections / epidemiology
  • Enterobacteriaceae Infections / microbiology
  • Escherichia coli / drug effects
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Escherichia coli / isolation & purification
  • Gene Expression
  • Hospitals
  • Humans
  • Isoenzymes / genetics
  • Isoenzymes / metabolism
  • Klebsiella pneumoniae / drug effects
  • Klebsiella pneumoniae / enzymology
  • Klebsiella pneumoniae / genetics*
  • Klebsiella pneumoniae / isolation & purification
  • Microbial Sensitivity Tests
  • Morganella morganii / drug effects
  • Morganella morganii / enzymology
  • Morganella morganii / genetics*
  • Morganella morganii / isolation & purification
  • Open Reading Frames
  • Plasmids / chemistry*
  • Plasmids / classification
  • Plasmids / metabolism
  • Sequence Analysis, DNA
  • beta-Lactamases / genetics*
  • beta-Lactamases / metabolism

Substances

  • Anti-Bacterial Agents
  • Carbapenems
  • DNA Transposable Elements
  • Isoenzymes
  • beta-lactamase KPC-2
  • beta-Lactamases