To determine whether fruit bats in Singapore have been exposed to filoviruses, we screened 409 serum samples from bats of 3 species by using a multiplex assay that detects antibodies against filoviruses. Positive samples reacted with glycoproteins from Bundibugyo, Ebola, and Sudan viruses, indicating filovirus circulation among bats in Southeast Asia.
Keywords: Bundibugyo virus; Ebola virus; Singapore; Southeast Asia; Sudan virus; filoviruses; fruit bats; serology; virus envelope glycoprotein; virus surveillance; viruses.