Cell sheets in cell therapies

Giles T S Kirby; Andrew Michelmore; Louise E Smith; Jason D Whittle; Robert D Short

doi:10.1016/j.jcyt.2017.11.004

Cell sheets in cell therapies

Cytotherapy. 2018 Feb;20(2):169-180. doi: 10.1016/j.jcyt.2017.11.004. Epub 2017 Dec 15.

Authors

Giles T S Kirby¹, Andrew Michelmore², Louise E Smith³, Jason D Whittle², Robert D Short⁴

Affiliations

¹ Future Industries Institute, University of South Australia, Mawson Lakes, Adelaide, Australia; Cooperative Research Centre for Cell Therapy Manufacturing, University of South Australia, Adelaide, Australia. Electronic address: giles.kirby@unisa.edu.au.
² Future Industries Institute, University of South Australia, Mawson Lakes, Adelaide, Australia; Cooperative Research Centre for Cell Therapy Manufacturing, University of South Australia, Adelaide, Australia; School of Engineering, University of South Australia, Mawson Lakes, Adelaide, Australia.
³ Future Industries Institute, University of South Australia, Mawson Lakes, Adelaide, Australia; Cooperative Research Centre for Cell Therapy Manufacturing, University of South Australia, Adelaide, Australia.
⁴ Future Industries Institute, University of South Australia, Mawson Lakes, Adelaide, Australia; Cooperative Research Centre for Cell Therapy Manufacturing, University of South Australia, Adelaide, Australia; Materials Science Institute and Department of Chemistry, University of Lancaster, City of Lancaster, United Kingdom.

PMID: 29254764
DOI: 10.1016/j.jcyt.2017.11.004

Abstract

This review aims to provide a broad introduction to the use of cell sheets and the role of materials in the delivery of cell sheets to patients within a clinical setting. Traditionally, cells sheets have been, and currently are, fabricated using established and accepted cell culture methods within standard formats (e.g., petri dishes) utilizing biological substrates. Synthetic surfaces provide a far more versatile system for culturing and delivering cell sheets. This has the potential to positively affect quality, and efficient, localized cell delivery has a significant impact on patient outcome and on the overall cost of goods. We highlight current applications of these advanced carriers and future applications of these surfaces and cell sheets with an emphasis both on clinical use and regulatory requirements.

Keywords: culture; plasma polymerization; regeneration; sheet; therapy.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Cell Culture Techniques / methods*
Cell- and Tissue-Based Therapy / methods*
Cells, Cultured
Humans
Stem Cell Transplantation