One-pot biosynthesis of silver nanoparticles using Iboza Riparia and Ilex Mitis for cytotoxicity on human embryonic kidney cells

Myalowenkosi I Sabela; Talent Makhanya; Suvardhan Kanchi; Mohd Shahbaaz; Danish Idress; Krishna Bisetty

doi:10.1016/j.jphotobiol.2017.12.010

One-pot biosynthesis of silver nanoparticles using Iboza Riparia and Ilex Mitis for cytotoxicity on human embryonic kidney cells

J Photochem Photobiol B. 2018 Jan:178:560-567. doi: 10.1016/j.jphotobiol.2017.12.010. Epub 2017 Dec 13.

Authors

Myalowenkosi I Sabela¹, Talent Makhanya², Suvardhan Kanchi³, Mohd Shahbaaz², Danish Idress⁴, Krishna Bisetty⁵

Affiliations

¹ Department of Chemistry, Durban University of Technology, P.O Box 1334, Durban 4000, South Africa. Electronic address: myalos@dut.ac.za.
² Department of Chemistry, Durban University of Technology, P.O Box 1334, Durban 4000, South Africa.
³ Department of Chemistry, Durban University of Technology, P.O Box 1334, Durban 4000, South Africa. Electronic address: ksuvardhan@gmail.com.
⁴ Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India.
⁵ Department of Chemistry, Durban University of Technology, P.O Box 1334, Durban 4000, South Africa. Electronic address: bisettyk@dut.ac.za.

PMID: 29253815
DOI: 10.1016/j.jphotobiol.2017.12.010

Abstract

Plant extracts continue gaining significant prominence in green synthesis of silver nanoparticles (AgNPs), due to their potential applications in nano-medicine and material engineering. This work reports on green synthesis of silver nanoparticles (AgNPs) from aqueous extracts of Iboza Riparia leaf and Ilex Mitis root bark with diterpenes (DTPs) and saponins (SPNs) as major components. After TEM, DLS, TGA/DSC, ATR, XRD and UV-Vis characterization, the relevant cytotoxicity studies were conducted with the MTT assay on human embryonic kidney cells (HEK293T) followed by antioxidant activity with ABTS. Overall, the AgNPs-DTPs (156nm) were found to be less toxic with 49.7% cell viability, while AgNPs-SPNs (50nm) and AgNPs-PVA (44nm) had cell viability of 40.8 and 28.0% respectively at 400μM. Based on the cytotoxicity and antioxidant activity, it is fair to report that these plant extracts have potential reducing and capping agents as they retain chemical properties on the surface of the nanoparticles.

Keywords: Antioxidant activity; Diterpenes; MTT assay; Saponins; Silver nanoparticles.

MeSH terms

Antioxidants / chemistry
Calorimetry, Differential Scanning
Cell Survival / drug effects
Diterpenes / chemistry
Diterpenes / isolation & purification
Green Chemistry Technology
HEK293 Cells
Humans
Ilex / chemistry*
Ilex / metabolism
Metal Nanoparticles / chemistry*
Metal Nanoparticles / toxicity
Particle Size
Plant Extracts / chemistry
Plant Leaves / chemistry
Plant Leaves / metabolism
Plant Roots / chemistry
Plant Roots / metabolism
Saponins / chemistry
Saponins / isolation & purification
Silver / chemistry*
Spectrophotometry, Ultraviolet
X-Ray Diffraction

Substances

Antioxidants
Diterpenes
Plant Extracts
Saponins
Silver