Long non-coding RNAs have been proved in tumorigenesis of various cancers, including osteosarcoma. However, the role of LOC730101 in cancer is rare to be reported, not mention to osteosarcoma. In current study, quantitative real-time PCR, was applied to evaluate the expression of LOC730101 under energy stress. The roles of LOC730101 in cell viability, apoptosis and tumor development under energy stress were analyzed by cell biological assays in vitro and in vivo. AMPK CA (continuously active) and Compound C (AMPK inhibitor) were employed to investigate the mechanism. And we further confirmed that compared with adjacent tissues, LOC730101 is remarkably increased in osteosarcoma in our fresh tissues. In addition, cellular function assays demonstrated that LOC730101 promotes cell viability, tumor development, and deficiency of LOC730101 promotes cell apoptosis under energy stress. Furthermore, we validated that up-regulated LOC730101 enhances cell viability through AMPK under energy stress. Taken together, our findings enlarged the knowledge about the roles of LOC730101 in osteosarcoma progression.
Keywords: AMPK; Cell proliferation; Energy stress; LOC730101; Osteosarcoma.
Copyright © 2017. Published by Elsevier Inc.