Dimethyloxalylglycine-embedded Poly(ε-caprolactone) Fiber Meshes Promote Odontoblastic Differentiation of Human Dental Pulp-derived Cells

Yeon-Jee Yoo; Joung-Hwan Oh; Qiankun Zhang; WooCheol Lee; Kyung Mi Woo

doi:10.1016/j.joen.2017.09.002

Dimethyloxalylglycine-embedded Poly(ε-caprolactone) Fiber Meshes Promote Odontoblastic Differentiation of Human Dental Pulp-derived Cells

J Endod. 2018 Jan;44(1):98-103.e1. doi: 10.1016/j.joen.2017.09.002.

Authors

Yeon-Jee Yoo¹, Joung-Hwan Oh², Qiankun Zhang², WooCheol Lee¹, Kyung Mi Woo³

Affiliations

¹ Department of Conservative Dentistry, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea.
² Department of Molecular Genetics, Dental Research Institute and BK21 Plus Program, School of Dentistry, Seoul National University, Seoul, Korea.
³ Department of Molecular Genetics, Dental Research Institute and BK21 Plus Program, School of Dentistry, Seoul National University, Seoul, Korea; Department of Pharmacology & Dental Therapeutics, School of Dentistry, Seoul National University, Seoul, Korea. Electronic address: kmwoo@snu.ac.kr.

PMID: 29246377
DOI: 10.1016/j.joen.2017.09.002

Abstract

Introduction: The in vivo effect of prolyl hydroxylase inhibitors on the regeneration of the pulp-dentin complex is unclear. The purpose of this study was to investigate the effect of dimethyloxalylglycine (DMOG)-embedded poly(ε-caprolactone) fiber (PCLF/DMOG) on odontoblastic differentiation of human dental pulp-derived cells (hDPCs) by transplantation of the dentin slice model.

Methods: The hDPCs were seeded onto electrospun PCLF and PCLF/DMOG in dentin slices and then transplanted into nude mice. The surface topography was evaluated for both PCLFs, and DMOG release from the PCLF/DMOG was examined. The effects of the PCLF/DMOG were assessed by histology and quantitative reverse transcription polymerase chain reaction.

Results: The PCLF/DMOG-treated dentin slices showed higher cellularity with a palisading arrangement of hDPCs and organized collagen fibers. We found that the PCLF/DMOG significantly stimulated the expression of vascular endothelial growth factor, dentin sialoprotein, and bone sialoprotein in the hDPCs (P < .05) and mouse vascular endothelial growth factor A, mouse platelet endothelial cell adhesion molecule 1, and mouse neurofilament light polypeptide in the surrounding host cells (P < .05).

Conclusions: These results show that PCLF/DMOG has potential in pulp-dentin complex regeneration by promoting odontoblastic differentiation of hDPCs and by enhancing host cell recruitment, angiogenesis, and neurogenesis through the released DMOG-mediated cell responses.

Keywords: Dimethyloxalylglycine; human dental pulp-derived cells; nanofibers; nude mice; odontoblastic differentiation; transplantation.

MeSH terms

Amino Acids, Dicarboxylic*
Animals
Cell Differentiation
Cells, Cultured
Dental Pulp / cytology*
Humans
Mice
Mice, Nude
Odontoblasts / cytology*
Polyesters*
Surgical Mesh

Substances

Amino Acids, Dicarboxylic
Polyesters
polycaprolactone
oxalylglycine