Here we report on the preparation of quasi-homogeneous fluorescence emission from graphene oxide (GO) film by modifying the local optical properties through the laser-induced fluorescence quenching effect, and the fabrication of single and multilayer micropatterns on quasi-homogeneous GO films. The modification is stemming from the photoreduction of GO, where the reduction degree and fluorescence intensity can be precisely tuned by changing the laser power and irradiation duration. This versatile approach with a mask-free feature can be readily used to fabricate various complex microstructures on quasi-homogeneous GO film from single layer to multilayer in vertical scale, as well as micrometers to centimeters in lateral scale. The micropatterns with varied optical properties are promising for applications in information storage, display technology, and optoelectronic devices.