Antifungal Activity of Ramulus cinnamomi Explored by ¹H-NMR Based Metabolomics Approach

Chunpeng Wan; Pei Li; Chuying Chen; Xuan Peng; Mingxi Li; Ming Chen; Junsong Wang; Jinyin Chen

doi:10.3390/molecules22122237

Antifungal Activity of Ramulus cinnamomi Explored by ¹H-NMR Based Metabolomics Approach

Molecules. 2017 Dec 15;22(12):2237. doi: 10.3390/molecules22122237.

Authors

Chunpeng Wan¹, Pei Li², Chuying Chen³, Xuan Peng⁴, Mingxi Li⁵, Ming Chen⁶, Junsong Wang⁷, Jinyin Chen^{8

9}

Affiliations

¹ Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits & Vegetables, Collaborative Innovation Center of Post-Harvest Key Technology and Quality Safety of Fruits and Vegetables, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China. chunpengwan@jxau.edu.cn.
² State key laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, China. lipei910425@126.com.
³ Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits & Vegetables, Collaborative Innovation Center of Post-Harvest Key Technology and Quality Safety of Fruits and Vegetables, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China. ccy0728@126.com.
⁴ Pingxiang University, Pingxiang 337055, China. pengx1104@163.com.
⁵ Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits & Vegetables, Collaborative Innovation Center of Post-Harvest Key Technology and Quality Safety of Fruits and Vegetables, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China. liming.xi@hotmail.com.
⁶ Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits & Vegetables, Collaborative Innovation Center of Post-Harvest Key Technology and Quality Safety of Fruits and Vegetables, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China. chenming@jxau.edu.cn.
⁷ Center for Molecular Metabolism, Nanjing University of Science and Technology, 200 Xiao Ling Wei, Nanjing 210014, China. wang.junsong@gmail.com.
⁸ Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits & Vegetables, Collaborative Innovation Center of Post-Harvest Key Technology and Quality Safety of Fruits and Vegetables, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China. jinyinchen@126.com.
⁹ Pingxiang University, Pingxiang 337055, China. jinyinchen@126.com.

Abstract

A ¹H nuclear magnetic resonance (NMR)-based approach to metabolomics combined bioassay was used to elucidate the antifungal activity of cinnamaldehyde (the main active compound of Ramulus cinnamomi) isolated from Ramulus cinnamomi (RC). Orthogonal signal correction partial least-squares discriminant analysis (OSC-PLS-DA) of NMR data was constructed to analyze all the P. italicum data acquired from the control and treatment groups at 4, 8, and 12 h. Metabolic profiles disclosed metabolic changes that were related to the antifungal effects of cinnamaldehyde against P. italicum including oxidative stress, disorder of energy metabolism, amino acids, and nucleic acids metabolism in treatment group. This integrated metabolomics approach provided an effective way to detect the antifungal effects of cinnamaldehyde against P. italicum dynamically.

Keywords: Penicillium italicum; Ramulus cinnamomi; antifungal activity; cinnamaldehyde; metabolomics analysis.

MeSH terms

Acrolein / analogs & derivatives*
Acrolein / chemistry
Acrolein / metabolism
Amino Acids / metabolism
Antifungal Agents / chemistry*
Antifungal Agents / metabolism*
Cinnamomum / chemistry*
Drugs, Chinese Herbal / chemistry*
Drugs, Chinese Herbal / metabolism
Energy Metabolism / drug effects
Humans
Magnetic Resonance Spectroscopy / methods*
Metabolome
Metabolomics
Nucleic Acids / metabolism
Oxidative Stress / drug effects

Substances

Amino Acids
Antifungal Agents
Drugs, Chinese Herbal
Nucleic Acids
Acrolein
cinnamaldehyde