Profiling of in vitro neurobiological effects and phenolic acids of selected endemic Salvia species

Ilkay Erdogan Orhan; Fatma Sezer Senol; Nilgun Ozturk; Galip Akaydin; Bilge Sener

doi:10.1016/j.foodchem.2011.11.119

Profiling of in vitro neurobiological effects and phenolic acids of selected endemic Salvia species

Food Chem. 2012 Jun 1;132(3):1360-1367. doi: 10.1016/j.foodchem.2011.11.119. Epub 2011 Dec 6.

Authors

Ilkay Erdogan Orhan¹, Fatma Sezer Senol², Nilgun Ozturk³, Galip Akaydin⁴, Bilge Sener²

Affiliations

¹ Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey; Pharmacognosy and Pharmaceutical Botany Unit, Faculty of Pharmacy, Eastern Mediterranean University, Gazimagosa, North Cyprus, Turkey. Electronic address: iorhan@gazi.edu.tr.
² Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey.
³ Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, 26470 Eskisehir, Turkey.
⁴ Department of Biology Education, Hacettepe University, 06800 Ankara, Turkey.

PMID: 29243623
DOI: 10.1016/j.foodchem.2011.11.119

Abstract

The ethyl acetate and methanol extracts from 16 Salvia L. species were screened for their inhibitory activity against acetylcholinesterase, butyrylcholinesterase, lipoxygenase, and tyrosinase; the enzymes linked to neurodegeneration. Their antioxidant activity was also tested using DPPH radical scavenging, metal-chelation, and ferric-reducing antioxidant power (FRAP) assays. Total flavonoid content of the extracts was determined by AlCl₃ reagent, while HPLC technique was applied for analysis of various phenolic acids in the extracts. The extracts exerted weak cholinesterase and tyrosinase inhibition, and remarkable inhibition against lipoxygenase (13.07±2.73-74.21±5.61%) at 100μgml^-1. The methanol extracts showed higher antioxidant activity in DPPH radical scavenging and FRAP assays. The extracts were analyzed for their gallic, protocateuchic, p-hydroxy-benzoic, vanillic, caffeic, chlorogenic, syringic, o- and p-coumaric, ferulic, rosmarinic, and tr-cinnamic acid contents and the methanol extract of Salvia ekimiana (153.50mg100g^-1) was revealed to be the richest in terms of rosmarinic acid.

Keywords: Antioxidant activity; Enzyme inhibition; HPLC; Lamiaceae; Phenolic acid; Salvia.