Determination of biogenic amines in Bokbunja (Rubus coreanus Miq.) wines using a novel ultra-performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry

Shaodong Jia; Yun Pyo Kang; Jeong Hill Park; Jeongmi Lee; Sung Won Kwon

doi:10.1016/j.foodchem.2011.11.069

Determination of biogenic amines in Bokbunja (Rubus coreanus Miq.) wines using a novel ultra-performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry

Food Chem. 2012 Jun 1;132(3):1185-1190. doi: 10.1016/j.foodchem.2011.11.069. Epub 2011 Nov 29.

Authors

Shaodong Jia¹, Yun Pyo Kang¹, Jeong Hill Park¹, Jeongmi Lee², Sung Won Kwon³

Affiliations

¹ College of Pharmacy, Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
² School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea. Electronic address: jlee0610@skku.edu.
³ College of Pharmacy, Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Republic of Korea. Electronic address: swkwon@snu.ac.kr.

PMID: 29243599
DOI: 10.1016/j.foodchem.2011.11.069

Abstract

A rapid and novel ultra-performance liquid chromatography/quadrupole-time of flight mass spectrometry (UPLC/Q-TOFMS) method was developed for the detection of seven biogenic amines in Korean Bokbunja wines. The biogenic amines were pre-column derivatised with dansyl chloride and subsequently extracted by a solid phase extraction cartridge. For the analysis, the dansylated amines were separated on an Acquity™ UPLC column and detected with a micrOTOF-Q II mass spectrometer. The method was validated in terms of linearity, sensitivity, precision and recovery. The coefficient of determination (R²) was higher than 0.994 for all calibration curves. The limit of detection was between 3 and 15ng/mL. The intra- and inter-day precisions were below 5.0% and 10.7%, respectively. The proposed method was successfully applied for the biogenic amine analysis of 18 commercially available Bokbunja wines. None of the biogenic amine levels found in Bokbunja wines surpassed the toxic levels reported in the literature.

Keywords: Biogenic amine; Bokbunja wine; Dansyl chloride; Q-TOFMS; UPLC.