Electroseparation of an antibacterial peptide fraction from snow crab by-products hydrolysate by electrodialysis with ultrafiltration membranes

Alain Doyen; Linda Saucier; Lucie Beaulieu; Yves Pouliot; Laurent Bazinet

doi:10.1016/j.foodchem.2011.11.059

Electroseparation of an antibacterial peptide fraction from snow crab by-products hydrolysate by electrodialysis with ultrafiltration membranes

Food Chem. 2012 Jun 1;132(3):1177-1184. doi: 10.1016/j.foodchem.2011.11.059. Epub 2011 Nov 18.

Authors

Alain Doyen¹, Linda Saucier², Lucie Beaulieu³, Yves Pouliot¹, Laurent Bazinet⁴

Affiliations

¹ Institute of Nutraceuticals and Functional Foods (INAF), Université Laval, Québec, QC, Canada G1V 0A6; Department of Food Science and Nutrition, Université Laval, Québec, QC, Canada G1V 0A6.
² Institute of Nutraceuticals and Functional Foods (INAF), Université Laval, Québec, QC, Canada G1V 0A6; Department of Animal Sciences, Université Laval, Québec, QC, Canada G1V 0A6.
³ Institute of Nutraceuticals and Functional Foods (INAF), Université Laval, Québec, QC, Canada G1V 0A6; Department of Biology, Chemistry and Geography, Université du Québec à Rimouski (UQAR), Rimouski, QC, Canada G5L 3A1.
⁴ Institute of Nutraceuticals and Functional Foods (INAF), Université Laval, Québec, QC, Canada G1V 0A6; Department of Food Science and Nutrition, Université Laval, Québec, QC, Canada G1V 0A6. Electronic address: Laurent.Bazinet@fsaa.ulaval.ca.

PMID: 29243598
DOI: 10.1016/j.foodchem.2011.11.059

Abstract

Recently, a snow crab by-products hydrolysate has demonstrated antibacterial properties due to a peptide with a molecular weight of about 800Da, but only at high concentration. Consequently, peptide hydrolysate has been fractionated to obtain peptides in a more purified form. The aim of this work was to separate a snow crab by-products hydrolysate by electrodialysis with ultrafiltration membranes (EDUF). EDUF, which allows separation of molecules according to their charges and molecular weights, was used to recover and concentrate the active antibacterial fraction. Two different ultrafiltration membranes (20 and 50kDa) and two electrical field strengths (2 and 14V/cm) were used as separation parameters. After EDUF separation, the 300-600Da peptide molecular weight range was the most recovered with an abundance of 94%. Moreover, fractionation at 14V/cm with ultrafiltration membranes of 50kDa allowed the recovery of an anionic fraction which showed antibacterial properties on Escherichia coli ATCC 25922 and Listeria innocua HPB 13.

Keywords: Antibacterial peptide fraction; Electric field strength; Electrodialysis with ultrafiltration membranes; Molecular weight cut-off; Peptide migration.