Zinc-Ligand Swapping Mediated Complex Formation and Sulfur Transfer between SufS and SufU for Iron-Sulfur Cluster Biogenesis in Bacillus subtilis

Takashi Fujishiro; Takuya Terahata; Kouhei Kunichika; Nao Yokoyama; Chihiro Maruyama; Kei Asai; Yasuhiro Takahashi

doi:10.1021/jacs.7b11307

Zinc-Ligand Swapping Mediated Complex Formation and Sulfur Transfer between SufS and SufU for Iron-Sulfur Cluster Biogenesis in Bacillus subtilis

J Am Chem Soc. 2017 Dec 27;139(51):18464-18467. doi: 10.1021/jacs.7b11307. Epub 2017 Dec 13.

Authors

Takashi Fujishiro¹, Takuya Terahata¹, Kouhei Kunichika¹, Nao Yokoyama¹, Chihiro Maruyama¹, Kei Asai², Yasuhiro Takahashi¹

Affiliations

¹ Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University , Shimo-ohkubo 255, Sakura-ku, Saitama 338-8570, Japan.
² Department of Bioscience, Graduate School of Agriculture, Tokyo University of Agriculture , Sakuragaoka 1-1-1, Setagaya-ku, Tokyo 156-8502, Japan.

PMID: 29235855
DOI: 10.1021/jacs.7b11307

Abstract

SufU is a zinc-containing protein involved in mobilization of sulfur from SufS for iron-sulfur cluster biogenesis of Bacillus subtilis. Structural basis for the sulfur transfer in SufS-SufU complex was revealed. A zinc-ligand exchange reaction upon SufS-SufU complexation provides a free thiol from Cys41 of SufU as a sulfur acceptor.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus subtilis / metabolism*
Bacterial Proteins / chemistry*
Bacterial Proteins / metabolism*
Cysteine / chemistry
Cysteine / metabolism
Iron-Sulfur Proteins / chemistry*
Iron-Sulfur Proteins / metabolism*
Ligands
Sulfur / metabolism*
Zinc / metabolism*

Substances

Bacterial Proteins
Iron-Sulfur Proteins
Ligands
Sulfur
Zinc
Cysteine