Chrysanthemum CmHSFA4 gene positively regulates salt stress tolerance in transgenic chrysanthemum

Plant Biotechnol J. 2018 Jul;16(7):1311-1321. doi: 10.1111/pbi.12871. Epub 2018 Jan 22.

Abstract

Salinity-induced Na+ toxicity and oxidative stress hamper plant growth. Here, we showed that expression of the chrysanthemum CmHSFA4, a homologue of the heat-shock factor AtHSFA4a, is inducible by salt and localizes to the nucleus. It is a transcription activator binding with HSE. Chrysanthemum overexpressing CmHSFA4 displayed enhanced salinity tolerance by limiting Na+ accumulation while maintaining K+ concentration, which is consistent with the up-regulation of ion transporters CmSOS1 and CmHKT2. Additionally, the transgenic plants reduced H2 O2 and O2∙- accumulation under salinity, which could be due to up-regulation of ROS scavenger activities such as SOD, APX and CAT as well as CmHSP70, CmHSP90. Together, these results suggest that CmHSFA4 conferred salinity tolerance in chrysanthemum as a consequence of Na+ /K+ ion and ROS homeostasis.

Keywords: CmHSFA4; ROS; chrysanthemum; ionic; overexpression; salinity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chlorophyll / metabolism
  • Chrysanthemum / genetics*
  • Gene Expression Regulation, Plant / genetics
  • Genes, Plant / genetics*
  • Heat Shock Transcription Factors / genetics*
  • Heat Shock Transcription Factors / physiology
  • Hydrogen Peroxide / metabolism
  • Phylogeny
  • Plant Proteins / genetics*
  • Plant Proteins / physiology
  • Plants, Genetically Modified / genetics
  • Plants, Genetically Modified / physiology
  • Potassium / metabolism
  • Reactive Oxygen Species / metabolism
  • Salt Tolerance / genetics
  • Salt-Tolerant Plants / genetics*
  • Sequence Alignment
  • Sodium / metabolism

Substances

  • Heat Shock Transcription Factors
  • Plant Proteins
  • Reactive Oxygen Species
  • Chlorophyll
  • Sodium
  • Hydrogen Peroxide
  • Potassium

Associated data

  • GENBANK/KY860577