New Transcriptional Reporters to Quantify and Monitor PPAR γ Activity

Séverine A Degrelle; Hussein Shoaito; Thierry Fournier

doi:10.1155/2017/6139107

New Transcriptional Reporters to Quantify and Monitor PPAR γ Activity

PPAR Res. 2017:2017:6139107. doi: 10.1155/2017/6139107. Epub 2017 Nov 1.

Authors

Séverine A Degrelle^{1

2

3}, Hussein Shoaito^{1

2}, Thierry Fournier^{1

2

3}

Affiliations

¹ INSERM, UMR-S1139, Faculté de Pharmacie de Paris, 75006 Paris, France.
² Université Paris Descartes, Sorbonne Paris Cité, 75006 Paris, France.
³ Fondation PremUp, 75006 Paris, France.

Abstract

The peroxisome-proliferator-activated-receptor-γ (PPARγ) is a member of the nuclear receptor superfamily that plays a critical role in diverse biological processes, including adipogenesis, lipid metabolism, and placental development. To study the activity of PPARγ, we constructed two new reporter genes: a fluorescent GFP-tagged histone-2B (PPRE-H2B-eGFP) and a secreted nanoluciferase (PPRE-pNL1.3[secNluc]). This study demonstrates their usage to monitor PPARγ activity in different cell types and screen for PPARγ's potential ligands.