Oncogenic non-coding RNA NEAT1 promotes the prostate cancer cell growth through the SRC3/IGF1R/AKT pathway

Int J Biochem Cell Biol. 2018 Jan:94:125-132. doi: 10.1016/j.biocel.2017.12.005. Epub 2017 Dec 7.

Abstract

Steroid receptor co-activator3 (SRC3) has been known to severe as an androgen receptor (AR) coactivator and is involved in the prostate cancer progression. Non-coding RNA (ncRNA) plays an important role in the cancer progression. However, the mechanism underlying the relationship between ncRNA and AR coactivators is still unclear. Here, we found a ncRNA, Nuclear Enriched Abundant Transcript 1 (NEAT1), was able to interact with SRC3 in the prostate cancer cell lines. NEAT1 can upregulate the AKT phosphorylation via a SRC3/IGF1R pathway. In function, NEAT1 promoted the prostate cancer cell growth through IGF1R/AKT signaling pathway. The NEAT1, SRC3, and IGF1R were highly expressed in the patients' samples of prostate cancer. Therefore, we found a novel SRC3 binding ncRNA that can promote the prostate cancer cell growth through SRC3/IGF1R/AKT pathway.

Keywords: IGF1R; NEAT1; Prostate cancer; SRC3.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Cell Proliferation
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Male
  • Middle Aged
  • Neoplasm Proteins / agonists
  • Neoplasm Proteins / metabolism
  • Neoplasm Staging
  • Nuclear Receptor Coactivator 3 / agonists*
  • Nuclear Receptor Coactivator 3 / metabolism
  • Phosphorylation
  • Prostate / enzymology
  • Prostate / metabolism
  • Prostate / pathology
  • Prostatic Neoplasms / enzymology
  • Prostatic Neoplasms / metabolism*
  • Prostatic Neoplasms / pathology
  • Protein Multimerization
  • Protein Processing, Post-Translational
  • Proto-Oncogene Proteins c-akt / agonists*
  • Proto-Oncogene Proteins c-akt / metabolism
  • RNA Interference
  • RNA, Long Noncoding / antagonists & inhibitors
  • RNA, Long Noncoding / metabolism*
  • RNA, Neoplasm / antagonists & inhibitors
  • RNA, Neoplasm / metabolism*
  • Receptor, IGF Type 1
  • Receptors, Somatomedin / agonists*
  • Receptors, Somatomedin / antagonists & inhibitors
  • Receptors, Somatomedin / genetics
  • Receptors, Somatomedin / metabolism
  • Signal Transduction*
  • Tissue Banks
  • Tumor Cells, Cultured

Substances

  • IGF1R protein, human
  • NEAT1 long non-coding RNA, human
  • Neoplasm Proteins
  • RNA, Long Noncoding
  • RNA, Neoplasm
  • Receptors, Somatomedin
  • NCOA3 protein, human
  • Nuclear Receptor Coactivator 3
  • Receptor, IGF Type 1
  • AKT1 protein, human
  • Proto-Oncogene Proteins c-akt