Synthesis and characterization of small-sized gold nanoparticles coated by bovine serum albumin (BSA) for cancer photothermal therapy

Selma M H Al-Jawad; Ali A Taha; Mohammad M F Al-Halbosiy; Lamyaa F A Al-Barram

doi:10.1016/j.pdpdt.2017.12.004

Synthesis and characterization of small-sized gold nanoparticles coated by bovine serum albumin (BSA) for cancer photothermal therapy

Photodiagnosis Photodyn Ther. 2018 Mar:21:201-210. doi: 10.1016/j.pdpdt.2017.12.004. Epub 2017 Dec 6.

Authors

Selma M H Al-Jawad¹, Ali A Taha², Mohammad M F Al-Halbosiy³, Lamyaa F A Al-Barram⁴

Affiliations

¹ Applied physics department, School of Applied Sciences, University of Technology, Baghdad, Iraq. Electronic address: Salma_aljawad@yahoo.com.
² Biotechnology department, School of Applied Sciences, University of Technology, Baghdad, Iraq.
³ Biotechnology Research Center, Al- Nahrain University, Baghdad, Iraq.
⁴ Department of Radiology Technology, College of Health and Medical Technology, Middle Technical University, Baghdad, Iraq.

PMID: 29223737
DOI: 10.1016/j.pdpdt.2017.12.004

Abstract

In the present study, small gold nanoparticles <5 nm coated with natural protein Bovine Serum Albumin (BSA) was synthesized and characterized using UV-vis spectrophotometer, Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM), zeta potential and scanning electron microscopy (SEM). Three types of cancer cell lines; Rhabdomyosarcoma (RD), Murine fibroblast (L20B) and RAW 264.7 monocyte-macrophage (MQ) were tested and treated by photothermal strategy, in vitro, by conjugating BSA-AuNPs complex of (0.125, 0.25, 0.5 and 1 mg/ml) concentrations with continuous low power laser irradiation, green (532 nm) and near-infrared (NIR) (800 nm) at 0.5, 1, 2 and 3 min, separately. Cytotoxicity effect was determined by MTT assay. The vital impact of photothermal technique has investigated at 1 mg/ml and 3 min irradiation period as identified in RD cell line in comparison with other types; where cytotoxicity more than 74% was reached. Prominent results were demonstrated in the green and NIR region by pH -induced aggregation effect of small nanoparticles inside the cancer cells, which make the small-sized BSA-AuNPs are promising agents for cancer photothermal therapy.

Keywords: BSA; Cytotoxicity; Photothermal therapy (PPT); Renal clearance; Small-sized gold nanoparticles.

MeSH terms

Animals
Cattle
Cell Line, Tumor
Drug Carriers / chemistry*
Gold / chemistry*
Hydrogen-Ion Concentration
Low-Level Light Therapy / methods*
Metal Nanoparticles / chemistry*
Mice
Microscopy, Electron, Transmission
RAW 264.7 Cells
Serum Albumin, Bovine / chemistry*

Substances

Drug Carriers
Serum Albumin, Bovine
Gold