Multiplicity-edited 1 H-1 H TOCSY experiment

Abstract

A ¹ H-¹ H total correlation spectroscopy (TOCSY) experiment incorporating ¹³ C multiplicity information is proposed. In addition, broadband ¹ H homodecoupling in the indirect dimension can be implemented using a perfect BIRD module that affords exclusive ¹ H chemical shift evolution with full decoupling of all heteronuclear and homonuclear (including ² J_HH ) coupling constants. As a complement to the normal TOCSY and the recent PSYCHE-TOCSY experiments, this novel multiplicity-edited TOCSY experiment distinguishes between CH/CH₃ (phased up) and CH₂ (phased down) cross-peaks, which facilitates resonance analysis and assignment.

Keywords: BIRD; TOCSY; broadband homodecoupling; carbon multiplicity; homodecoupled TOCSY; multiplicity-edited TOCSY; perfect BIRD; pure shift NMR.