GLUT2-Expressing Neurons as Glucose Sensors in the Brain: Electrophysiological Analysis

Gwenaël Labouèbe; Bernard Thorens; Christophe Lamy

doi:10.1007/978-1-4939-7507-5_19

GLUT2-Expressing Neurons as Glucose Sensors in the Brain: Electrophysiological Analysis

Methods Mol Biol. 2018:1713:255-267. doi: 10.1007/978-1-4939-7507-5_19.

Authors

Gwenaël Labouèbe¹, Bernard Thorens², Christophe Lamy³

Affiliations

¹ Center for Integrative Genomics, University of Lausanne, Genopode Building, Lausanne, 1015, Switzerland. Gwenael.Labouebe@unil.ch.
² Center for Integrative Genomics, University of Lausanne, Genopode Building, Lausanne, 1015, Switzerland.
³ Department of Medicine, University of Fribourg, Fribourg, 1700, Switzerland.

PMID: 29218531
DOI: 10.1007/978-1-4939-7507-5_19

Abstract

Brain glucose sensing plays an essential role in the regulation of energy homeostasis. Recent publications report that neurons expressing glucose transporter GLUT2 act as glucose sensors in different regions of the brain and contribute to the control of glucose homeostasis and feeding behavior. In this chapter we describe the methods used to explore glucose sensing in genetically tagged GLUT2-expressing neurons with slice electrophysiology.

Keywords: Electrophysiology; GLUT2; Glucose sensing; Juxtacellular; Mice; NTS; Neurons; PVT; Whole-cell.

MeSH terms

Animals
Brain / physiology*
Electrophysiological Phenomena*
Gene Expression*
Glucose / metabolism*
Glucose Transporter Type 2 / genetics*
Glucose Transporter Type 2 / metabolism
Male
Mice
Molecular Imaging
Neurons / physiology*
Patch-Clamp Techniques

Substances

Glucose Transporter Type 2
Glucose