Glucose Transport: Methods for Interrogating GLUT4 Trafficking in Adipocytes

Dougall M Norris; Tom A Geddes; David E James; Daniel J Fazakerley; James G Burchfield

doi:10.1007/978-1-4939-7507-5_15

Glucose Transport: Methods for Interrogating GLUT4 Trafficking in Adipocytes

Methods Mol Biol. 2018:1713:193-215. doi: 10.1007/978-1-4939-7507-5_15.

Authors

Dougall M Norris^{1

2}, Tom A Geddes^{1

2}, David E James^{1

3

2}, Daniel J Fazakerley^{1

2}, James G Burchfield^{4

5}

Affiliations

¹ School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW, 2006, Australia.
² The Charles Perkins Centre, The University of Sydney, Sydney, 2006, NSW, Australia.
³ Sydney Medical School, The University of Sydney, Sydney, NSW, 2006, Australia.
⁴ School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW, 2006, Australia. james.burchfield@sydney.edu.au.
⁵ The Charles Perkins Centre, The University of Sydney, Sydney, 2006, NSW, Australia. james.burchfield@sydney.edu.au.

PMID: 29218527
DOI: 10.1007/978-1-4939-7507-5_15

Abstract

In this chapter we detail methods for the systematic dissection of GLUT4 trafficking. The methods described have been optimized for cultured 3T3-L1 adipocytes, but can be readily adapted to other cell types.

Keywords: Calibration; Fluorescence; Fractionation; GLUT4; GSV; Glucose transport; Imaging; Immunoprecipitation; Kinetics; Trafficking.

MeSH terms

3T3-L1 Cells
Adipocytes / metabolism*
Animals
Biological Transport
Fluorescent Antibody Technique
Glucose / metabolism*
Glucose Transporter Type 4 / metabolism*
Mice
Molecular Imaging
Protein Transport

Substances

Glucose Transporter Type 4
Glucose