Effect of lncRNA HOXA11-AS1 on adipocyte differentiation in human adipose-derived stem cells

Biochem Biophys Res Commun. 2018 Jan 8;495(2):1878-1884. doi: 10.1016/j.bbrc.2017.12.006. Epub 2017 Dec 5.

Abstract

Aims: To determine the role of lncRNA HOXA11-AS1 on adipocyte differentiation.

Methods: Human adipose-derived stem cells (hADSCs) were isolated from adipose tissues of patients and cultured in vitro, followed by knockdown of HOXA11-AS1. Then, adipocyte differentiation and expression of adipogenic-related genes (CEBP-α, DGAT2, CIDEC, and perilipin) were measured by RT-qPCR and Western blot.

Results: We demonstrated that knockdown of HOXA11-AS1 inhibited adipocyte differentiation, leading to suppression of adipogenic-related gene (CEBP-α, DGAT2, CIDEC, and perilipin) transcription, as well as decreased lipid accumulation in hADSCs. In addition, lncRNA HOXA11-AS1 was highly expressed in obese patients and significantly increased during the process of adipocyte differentiation.

Conclusion: The results provide new insight into the molecular mechanism by which lncRNA HOXA11-AS1 is involved in adipogenesis and may have implications for the treatment of obesity and associated disorders.

Keywords: Adipogenesis; Adipogenic-related gene; Human adipose-derived stem cells (hADSCs); Long non-coding RNA; Obesity; lncRNA HOXA11-AS1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / metabolism*
  • Adipocytes / pathology
  • Adipogenesis*
  • Adipose Tissue / growth & development*
  • Adipose Tissue / pathology
  • Cell Differentiation / physiology
  • Cells, Cultured
  • Homeodomain Proteins / metabolism*
  • Humans
  • Obesity / metabolism*
  • RNA, Long Noncoding / metabolism*
  • Stem Cells / pathology
  • Stem Cells / physiology*

Substances

  • HOXA11 protein, human
  • Homeodomain Proteins
  • RNA, Long Noncoding