Hollow mesoporous zirconia delivery system for biomineralization precursors

Xue-Qing Huang; Hong-Ye Yang; Tao Luo; Cui Huang; Franklin R Tay; Li-Na Niu

doi:10.1016/j.actbio.2017.11.049

Hollow mesoporous zirconia delivery system for biomineralization precursors

Acta Biomater. 2018 Feb:67:366-377. doi: 10.1016/j.actbio.2017.11.049. Epub 2017 Dec 6.

Authors

Xue-Qing Huang¹, Hong-Ye Yang², Tao Luo³, Cui Huang², Franklin R Tay⁴, Li-Na Niu⁵

Affiliations

¹ Department of Prosthodontics, Guanghua School and Hospital of Stomatology & Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, PR China.
² Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, Hubei, PR China.
³ Stomatology Hospital of Guangzhou Medical University, Guangzhou, Guangdong, PR China.
⁴ College of Graduate Studies, Augusta University, Augusta, GA, USA. Electronic address: ftay@augusta.edu.
⁵ State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, PR China. Electronic address: niulina831013@126.com.

PMID: 29208555
DOI: 10.1016/j.actbio.2017.11.049

Abstract

Strategies based on the combination of nanocarrier delivery systems and scaffolds provide bone tissue engineering scaffolds with multifunctional capability. Zirconia, a biocompatible ceramic commonly used in orthopedic and dental implants, was used to synthesize hollow mesoporous nanocapsules for loading, storage and sustained release of a novel polyamine-stabilized liquid precursor phase of amorphous calcium phosphate (PAH-ACP) for collagen biomineralization and bone marrow stromal cells osteoinduction. Hollow mesoporous zirconia (hmZrO₂) nanocapsules loaded with biomimetic precursors exhibited pH-sensitive release capability and good biocompatibility. The PAH-ACP released from loaded hmZrO₂ still retained the ability to infiltrate and mineralize collagen fibrils as well as exhibited osteoinductivity. A collagen scaffold blended with PAH-ACP@hmZrO₂ supplement and stem cells may be a promising tool for bone tissue engineering.

Statement of significance: The advent of nanotechnology has catalyzed the development of bone tissue engineering strategies based on the combination of nanocarrier delivery systems and scaffolds, which provide distinct advantages, including the possibilities of sustained release and protection of the bioactive agents, site-specific pharmacological effects and reduction of side effects. Herein, hollow mesoporous zirconia (hmZrO₂) nanocapsules with pH-sensitive capacity were synthesized for loading, storage and sustained release of a novel polyamine-stabilized liquid precursor phase of ACP (PAH-ACP). The loaded nanocapsules show good biocompatibility and demonstrate bioactivities for collagen biomineralization and bone marrow stromal cells osteoinduction. Our results may offer a promising tool for designing bone tissue engineering "cocktail therapy" involving seeding scaffolds with biomineralization precursors loaded hmZrO₂ supplement and stem cells.

Keywords: Biomineralization; Nanocapsules; Osteoinduction; Tissue engineering; Zirconia.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Calcification, Physiologic / drug effects*
Cattle
Drug Delivery Systems*
Female
Hydrogen-Ion Concentration
Mesenchymal Stem Cells / cytology
Mesenchymal Stem Cells / drug effects
Mesenchymal Stem Cells / metabolism
Mice
Mice, Inbred C57BL
Porosity
RAW 264.7 Cells
Spectrometry, X-Ray Emission
X-Ray Diffraction
Zirconium / pharmacology*

Substances

Zirconium
zirconium oxide