Piperine induces osteoblast differentiation through AMPK-dependent Runx2 expression

Do-Young Kim; Eun-Jung Kim; Won-Gu Jang

doi:10.1016/j.bbrc.2017.11.200

Piperine induces osteoblast differentiation through AMPK-dependent Runx2 expression

Biochem Biophys Res Commun. 2018 Jan 1;495(1):1497-1502. doi: 10.1016/j.bbrc.2017.11.200. Epub 2017 Dec 5.

Authors

Do-Young Kim¹, Eun-Jung Kim², Won-Gu Jang³

Affiliations

¹ Department of Biotechnology, College of Engineering, Daegu University, Gyeongbuk, 38453, Republic of Korea; Research Institute of Anti-Aging, Daegu University, Gyeongbuk, 38453, Republic of Korea. Electronic address: hajin912@gmail.com.
² Research Institute of Anti-Aging, Daegu University, Gyeongbuk, 38453, Republic of Korea; Department of Immunology, Kyungpook National University School of Medicine, Daegu, 41944, Republic of Korea. Electronic address: ejkim4164@knu.ac.kr.
³ Department of Biotechnology, College of Engineering, Daegu University, Gyeongbuk, 38453, Republic of Korea; Research Institute of Anti-Aging, Daegu University, Gyeongbuk, 38453, Republic of Korea. Electronic address: jangwg@daegu.ac.kr.

PMID: 29203239
DOI: 10.1016/j.bbrc.2017.11.200

Abstract

Piperine is an alkaloid responsible for the pungency of black pepper and long pepper. It is reported to have various biological actions such as anti-oxidative and anti-inflammatory, and aids cancer prevention. Antioxidants have been shown to promote osteoblast differentiation. However, osteoblast differentiation by piperine has not yet been elucidated. Piperine-induced expression of the osteogenic genes such as distal-less homeobox 5 (Dlx5), inhibitor of DNA binding-1 (Id1), and runt-related transcription factor 2 (Runx2) was investigated using RT-PCR. In addition, alkaline phosphatase (ALP) activity and mineralization was found to be increased by piperine treatment. Finally, we confirmed that piperine induced phosphorylation of AMPK in MC3T3-E1 cells. Taken together, these results demonstrate that piperine enhance osteoblast differentiation through AMPK phosphorylation in MC3T3-E1 cells.

Keywords: AMP-activated protein kinase (AMPK); C3H10T1/2; MC3T3-E1; Osteogenic differentiation; Piperine.

MeSH terms

3T3 Cells
AMP-Activated Protein Kinase Kinases
Alkaloids / administration & dosage*
Animals
Antioxidants / administration & dosage
Benzodioxoles / administration & dosage*
Cell Differentiation / drug effects
Cell Differentiation / physiology*
Core Binding Factor Alpha 1 Subunit / metabolism*
Dose-Response Relationship, Drug
Gene Expression Regulation, Developmental / drug effects
Gene Expression Regulation, Developmental / physiology
Mice
Osteoblasts / cytology
Osteoblasts / drug effects
Osteoblasts / physiology*
Osteogenesis / drug effects
Osteogenesis / physiology*
Piperidines / administration & dosage*
Polyunsaturated Alkamides / administration & dosage*
Protein Kinases / metabolism*

Substances

Alkaloids
Antioxidants
Benzodioxoles
Core Binding Factor Alpha 1 Subunit
Piperidines
Polyunsaturated Alkamides
Runx2 protein, mouse
Protein Kinases
AMP-Activated Protein Kinase Kinases
piperine