One-pot fabrication of chitin-shellac composite microspheres for efficient enzyme immobilization

Shuang Mei; Pingping Han; Hong Wu; Jiafu Shi; Lei Tang; Zhongyi Jiang

doi:10.1016/j.jbiotec.2017.11.015

One-pot fabrication of chitin-shellac composite microspheres for efficient enzyme immobilization

J Biotechnol. 2018 Jan 20:266:1-8. doi: 10.1016/j.jbiotec.2017.11.015. Epub 2017 Dec 2.

Authors

Shuang Mei¹, Pingping Han¹, Hong Wu², Jiafu Shi³, Lei Tang¹, Zhongyi Jiang¹

Affiliations

¹ Key Laboratory for Green Chemical Technology of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, China.
² Key Laboratory for Green Chemical Technology of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, China; Tianjin Key Laboratory of Membrane Science and Desalination Technology, Tianjin University, Tianjin 300072, China. Electronic address: wuhong@tju.edu.cn.
³ Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, China; School of Environment Science and Engineering, Tianjin University, Tianjin 300072, China.

PMID: 29199127
DOI: 10.1016/j.jbiotec.2017.11.015

Abstract

In this study, all-natural composite microspheres were fabricated through adding shellac into chitin solution followed by self-assembly via thermally-induced phase separation. The pore structure of the composite microspheres was altered into wedge-shape from ink-bottle-shape of the chitin microspheres, whereas, the crystalline structure of these two kinds of microspheres remained unaltered. The as-fabricated chitin-shellac composite microspheres were used for enzyme immobilization through adsorption. And yeast alcohol dehydrogenase (YADH) was chosen as the model enzyme, which is a multimer consisting of 4 subunits. The loading capacity of the as-prepared composite microspheres was up to 79.0mg/g (enzyme/carrier). The immobilized enzyme exhibited a comparable catalytic activity compared to its free counterpart and maintained 49.3% of its initial activity after 54days' storage at 4°C while the free enzyme lost all its activity.

Keywords: Chitin; Composite microspheres; Enzyme immobilization; One-pot fabrication; Shellac.

MeSH terms

Alcohol Dehydrogenase / chemistry*
Chitin / chemistry*
Enzymes, Immobilized / chemistry*
Microspheres*
Resins, Plant / chemistry*
Saccharomyces cerevisiae / enzymology*
Saccharomyces cerevisiae Proteins / chemistry*

Substances

Enzymes, Immobilized
Resins, Plant
Saccharomyces cerevisiae Proteins
Chitin
shellac
Alcohol Dehydrogenase