Comparison of Abbott Architect®, Siemens Immulite®, and Diasorin Liaison® for determination of Epstein-Barr virus serological diagnosis

Diagn Microbiol Infect Dis. 2018 Feb;90(2):96-101. doi: 10.1016/j.diagmicrobio.2017.10.020. Epub 2017 Nov 7.

Abstract

This study compared the performance of 3 automated immunoassays, Architect® (Abbott), Immulite® (Siemens) and Liaison® (Diasorin), for Epstein-Barr virus (EBV) serology. Ninety-one serum samples collected in Amiens University Hospital were analyzed for the presence of Viral Capsid Antigen (VCA) IgG and IgM and Epstein-Barr Nuclear Antigen (EBNA) IgG. The agreement between the 3 assays was calculated for each marker individually and for determination of the EBV profile, based on interpretation of the combination of these 3 EBV markers. Although similar results were obtained with Architect® and Liaison®, several discordant results were observed with Immulite®, particularly for EBNA IgG. A large number of EBNA IgG-positive results were observed, which interfered with interpretation of the EBV profile. In contrast, Immulite® performed similarly to the 2 other assays for detection of VCA IgM.

Keywords: Architect; EBV; Epstein–Barr virus; Immulite; Infectious mononucleosis; Liaison.

Publication types

  • Comparative Study

MeSH terms

  • Antibodies, Viral / blood*
  • Antigens, Viral / blood*
  • Epstein-Barr Virus Infections / diagnosis*
  • Epstein-Barr Virus Infections / virology
  • Herpesvirus 4, Human / immunology*
  • Herpesvirus 4, Human / isolation & purification
  • Humans
  • Immunoassay / methods*
  • Immunoglobulin G / blood
  • Immunoglobulin M / blood
  • Retrospective Studies
  • Sensitivity and Specificity
  • Virology

Substances

  • Antibodies, Viral
  • Antigens, Viral
  • Immunoglobulin G
  • Immunoglobulin M