Circulating levels of specific members of chromosome 19 microRNA cluster are associated with preeclampsia development

Arch Gynecol Obstet. 2018 Feb;297(2):365-371. doi: 10.1007/s00404-017-4611-6. Epub 2017 Dec 1.

Abstract

Purpose: To perform serum microRNA expression profiling to identify members of chromosome 19 miRNA cluster involved in preeclampsia development.

Methods: Serum chromosome 19 miRNA cluster microRNA expression profiling was evaluated at 12, 16, and 20 gestational weeks and at the time of preeclampsia diagnosis, in women who developed preeclampsia (WWD-PE; n = 16) and controls (n = 18) using TaqMan low density array plates.

Results: A total of 51 chromosome 19 microRNA cluster members were evaluated. The circulating hsa-miRs 512-3p, 518f-3p, 520c-3p, and 520d-3p, were differentially expressed between groups (P < 0.05). Compared with controls, serum levels of hsa-miR-518f-3p at 20 GW were useful for identifying WWD-Mild-PE (P = 0.035) and WWD-Severe-PE (P = 0.007).

Conclusions: Serum hsa-miRs 512-3p, 518f-3p, 520c-3p, and 520d-3p, are differentially expressed between WWD-PE and controls and their role in the development of preeclampsia should be investigated further.

Keywords: Biomarkers; MicroRNA; Placenta; Preeclampsia; Signaling pathway.

MeSH terms

  • Case-Control Studies
  • Chromosomes, Human, Pair 19*
  • Female
  • Gene Expression Profiling*
  • Gene Expression Regulation, Developmental
  • Humans
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism*
  • Placenta / metabolism*
  • Pre-Eclampsia / blood*
  • Pre-Eclampsia / genetics
  • Pre-Eclampsia / metabolism
  • Pregnancy

Substances

  • MIRN512 microRNA, human
  • MicroRNAs