Arabidopsis calcium-dependent protein kinase AtCPK1 plays a positive role in salt/drought-stress response

Biochem Biophys Res Commun. 2018 Mar 25;498(1):92-98. doi: 10.1016/j.bbrc.2017.11.175. Epub 2017 Dec 2.

Abstract

The calcium-dependent protein kinases (CDPKs) play vital roles in plant response to various environmental stimuli. Here, we investigated the function of Arabidopsis AtCPK1 in response to salt and drought stress. The loss-of-function cpk1 mutant displayed hypersensitive to salt and drought stress, whereas overexpressing AtCPK1 in Arabidopsis plants significantly enhanced the resistance to salt or drought stress. The reduced or elevated tolerance of cpk1 mutant and AtCPK1-overexpressing lines was confirmed by the changes of proline, malondialdehyde (MDA) and H2O2. Real-time PCR analysis revealed that the expression of several stress-inducible genes (RD29A, COR15A, ZAT10, APX2) down-regulated in cpk1 mutant and up-regulated in AtCPK1-overexpressing plants. These results are likely to indicate that AtCPK1 positively regulates salt and drought stress in Arabidopsis.

Keywords: Arabidopsis; AtCPK1; CDPKs; Drought stress; Salt stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / drug effects
  • Arabidopsis / enzymology*
  • Arabidopsis / genetics
  • Arabidopsis / physiology*
  • Arabidopsis Proteins / metabolism*
  • Droughts*
  • Gene Expression Regulation, Plant / drug effects
  • Hydrogen Peroxide / metabolism
  • Malondialdehyde / metabolism
  • Mutation / genetics
  • Phenotype
  • Plants, Genetically Modified
  • Polyethylene Glycols / pharmacology
  • Proline / metabolism
  • Protein Kinases / metabolism*
  • Sodium Chloride / pharmacology*
  • Stress, Physiological / drug effects*
  • Transcription, Genetic / drug effects

Substances

  • Arabidopsis Proteins
  • Polyethylene Glycols
  • Sodium Chloride
  • Malondialdehyde
  • Proline
  • Hydrogen Peroxide
  • Protein Kinases
  • CPK1 protein, Arabidopsis